Genome-wide analyses of Escherichia coli gene expression responsive to the BaeSR two-component regulatory system
Genome-wide analyses of Escherichia coli gene expression responsive to the BaeSR two-component regulatory system
复制标题
DOI：
10.1128/jb.187.5.1763-1772.2005
复制
发表时间：
2005-03-01
影响因子：
3.2
通讯作者：
Yamaguchi, A
中科院分区：
文献类型：
--
作者：
Nishino, K;Honda, T;Yamaguchi, A
BaeSR双组分调控系统控制着在大肠杆菌(S.Nagakubo，K.Nishino，T.Hirata和A.Yamaguchi，J.细菌素)中导致耐药性的出口基因的表达。184：4161-4167,2002；N.Baranova和H.Nikaido，J.细菌学。184：4168-4176,2002)。为了了解BaeSR调控的全貌，进行了Baer过量生产影响的DNA微阵列分析。Baer的过量生产激活了59个与双组分信号转导、趋化反应、鞭毛生物合成、麦芽糖转运和多药转运相关的基因，而Baer的过度生产也抑制了ibpA和ibpB基因的表达。用实时荧光定量逆转录-聚合酶链式反应(RT-PCR)技术观察基因表达水平的变化。BaeSR缺失后，59个Baer激活基因中有15个表达水平下降。在吲哚诱导的11个基因中，有10个基因需要BaeSR系统才能诱导。表达数据集的组合揭示了Baer结合位点序列基序5‘-TTTTTCTCCATDATTGGC-3’(其中D为G、A或T)。Baer过量生产上调的几个基因，包括麦芽糖运输、趋化反应和鞭毛生物合成的基因，需要一个完整的PhoBR或CreBC双组分调控系统才能上调。这些数据表明，BaeSR、PhoBR和CreBC两组分监管系统之间存在交叉监管。这样的全球分析应该会揭示BaeSR系统的监管网络。
The BaeSR two-component regulatory system controls expression of exporter genes conferring drug resistance in Escherichia coli (S. Nagakubo, K. Nishino, T. Hirata, and A. Yamaguchi, J. Bacteriol. 184:4161-4167, 2002; N. Baranova and H. Nikaido, J. Bacteriol. 184:4168-4176, 2002). To understand the whole picture of BaeSR regulation, a DNA microarray analysis of the effect of BaeR overproduction was performed. BaeR overproduction activated 59 genes related to two-component signal transduction, chemotactic responses, flagellar biosynthesis, maltose transport, and multidrug transport, and BaeR overproduction also repressed the expression of the ibpA and ibpB genes. All of the changes in the expression levels were also observed by quantitative real-time reverse transcription-PCR analysis. The expression levels of 15 of the 59 BaeR-activated genes were decreased by deletion of baeSR. Of 11 genes induced by indole (a putative inducer of the BaeSR system), 10 required the BaeSR system for induction. Combination of the expression data sets revealed a BaeR-binding site sequence motif, 5'-TTTTTCTCCATDATTGGC-3' (where D is G, A, or T). Several genes up-regulated by BaeR overproduction, including genes for maltose transport, chemotactic responses, and flagellar biosynthesis, required an intact PhoBR or CreBC two-component regulatory system for up-regulation. These data indicate that there is cross-regulation among the BaeSR, PhoBR, and CreBC two-component regulatory systems. Such a global analysis should reveal the regulatory network of the BaeSR system.