Tick troponin I-like molecule is a potent inhibitor for angiogenesis
Tick troponin I-like molecule is a potent inhibitor for angiogenesis
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DOI：
10.1016/j.mvr.2006.02.003
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Fujisaki, Kozo
中科院分区：
文献类型：
--
作者：
Fukumoto, Shinya;Sakaguchi, Takeshi;Fujisaki, Kozo
这是首次发现粘蜱唾液中的抗血管生成分子。在我们之前的研究中，我们鉴定了硬蜱长角血蜱的肌钙蛋白i样分子（htni），它是多种病原体的载体。为了研究其对血管生成的潜在抑制作用，我们在大肠杆菌中表达并纯化了重组HLTnI。在血管内皮生长因子（VEGF）竞争血管生成实验中，重组htni显著抑制体外人血管内皮细胞（H-UVEC）的毛细血管形成。抑制作用呈剂量依赖性，IC50为18.95 nM。这些结果表明HLTnI是一种有效的血管生成抑制剂。(c) 2006爱思唯尔公司版权所有。
This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (H-UVEC) in vitro. The inhibition was dose-dependent with an IC50 of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor. (c) 2006 Elsevier Inc. All rights reserved.