Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells
Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells
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DOI：
10.1007/s004410051042
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发表时间：
1998-04-01
影响因子：
3.6
通讯作者：
Murphy, PM
中科院分区：
文献类型：
--
作者：
Becker, EL;Forouhar, FA;Murphy, PM
甲酰肽受体(Fpr)以前只在多形核白细胞和单核/巨噬细胞上发现，它对合成的N-甲酰寡肽和细菌产生的N-甲酰寡肽都有反应。我们克隆了人FPR的基因，并制备了针对合成的11个氨基酸的多肽的兔多克隆抗血清。我们现在已经广泛地表征并使用该抗体来检测正常人体组织和细胞类型上的FPR。受体抗原存在于一些上皮细胞上，特别是那些具有分泌功能的细胞上，以及一些内分泌细胞上，如甲状腺的滤泡细胞和肾上腺的皮质细胞。肝细胞和枯否细胞阳性。平滑肌和血管内皮细胞也普遍呈阳性。在大脑和脊髓中，运动、感觉和小脑系统的神经元，以及副交感和交感系统的神经元呈阳性染色。这些数据表明，推定的FPR内源性激动剂或抗原性相似的受体与神经肌肉、血管、内分泌和免疫系统中的细胞靶点发生反应。
The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptide corresponding to the deduced carboxy-terminus has been produced. We have now extensively characterized and used the antibody to detect FPR on normal human tissues and cell types. The receptor antigen is present on some epithelial cells, especially those with a secretory function, and on some endocrine cells, e,g., follicular cells of the thyroid and cortical cells of the adrenal. Liver hepatocytes and Kupffer cells are positive. Smooth muscle and endothelial cells are also generally positive. In the brain and spinal cord, the neurons of the motor, sensory, and cerebellar systems, and those of the parasympathetic and sympathetic systems stain positively. These data suggest that the putative endogenous agonist for FPR or an antigenically similar receptor reacts with cellular targets in the neuromuscular, vascular, endocrine, and immune systems.