Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS
Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS
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DOI：
10.2217/fmb-2018-0015
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发表时间：
2018-07-01
影响因子：
3.1
通讯作者：
Hsueh, Po-Ren
中科院分区：
文献类型：
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作者：
Chen, Shey-Ying;Lee, Hao;Hsueh, Po-Ren
目的：我们评估了基质辅助激光解吸/电离飞行时间质谱仪的银葡萄球菌特异性诊断谱，以准确鉴定这种新型细菌。材料与方法：根据crtM基因阴性和存在特定序列类型鉴定银色葡萄球菌。通过基质辅助激光解吸/电离飞行时间质谱仪结果的聚类分析和25 S的ClinProTools软件生成分类模型。argenteus和25株对甲氧西林敏感的S.金黄色葡萄球菌（MSSA）。对72 S的分类模型的性能进行了验证。argenteus和72个MSSA分离株。结果：通过聚类分析和分类模型，对72个S. 72例MSSA银染标本化学提取法的准确性为100.0%，直接涂片法的敏感性为87.5%，特异性为100.0%。结论：该分类模型能准确区分S.来自MSSA的Argenteus。
Aim: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. Conclusion: The classification model could accurately differentiate S. argenteus from MSSA.