Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation
Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation
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DOI：
10.1073/pnas.96.4.1457
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发表时间：
1999-02-16
影响因子：
11.1
通讯作者：
Tokuhisa, T
中科院分区：
文献类型：
--
作者：
Kobayashi, K;Hatano, M;Tokuhisa, T
凋亡抑制因子（IAP）蛋白形成高度保守的基因家族，其防止响应于多种刺激的细胞死亡。在这里，我们描述了一个新定义的小鼠IAP，指定Tiap，这被证明是一个小鼠同源的人生存素的基础上进行序列比较。TIAP具有一个杆状病毒IAP重复序列，并且缺少C-末端的RING指基序。TIAP与caspase 3的加工形式相互作用并抑制caspase诱导的细胞死亡。组织学检查显示TIAP在成年小鼠的胸腺、睾丸和肠等生长组织以及胚胎的许多组织中表达。在体外实验中，用抗CD 3抗体或ConA激活的脾T细胞可诱导TIAP的表达，同步化的NM 3 T3细胞在S期至G（2）/M期TIAP的表达上调。我们提出，在细胞增殖过程中，细胞保护活性可能会增加诱导型IAP，如TIAP。
The inhibitor of apoptosis (IAP) proteins form a highly conserved gene family that prevents cell death in response to a variety of stimuli. Herein we describe a newly defined murine IAP, designated Tiap, that proved to be a murine homologue of human survivin based on sequence comparison. TIAP has one baculovirus IAP repeat and lacks a C-terminaI RING finger motif. TIAP interacted with the processed form of caspase 3 and inhibited caspase-induced cell death. Histological examinations revealed that TIAP is expressed in growing tissues such as thymus, testis, and intestine of adult mice and many tissues of embryos. In in vitro studies, TIAP was induced in splenic T cells activated with anti-CD3 antibody or Con A, and the expression of TIAP was up-regulated in synchronized NM 3T3 cells at S to G(2)/M phase of the cell cycle. We propose that during cell proliferation, cellular protective activity may be augmented with inducible IAPs such as TIAP.