Molecular probes define different regions of the mouse t complex
Molecular probes define different regions of the mouse t complex
复制标题
分子探针定义小鼠 t 复合体的不同区域
DOI：
10.1016/0092-8674(85)90309-5
复制
发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
L. Silver
中科院分区：
文献类型：
--
作者：
H. Fox;G. Martin;M. Lyon;B. Herrmann;A. Frischauf;H. Lehrach;L. Silver
从小鼠17号染色体近端部分的显微切割片段获得的四个基因组克隆已被用于鉴定一系列t-单倍型特异性限制性片段。它们的特异性是由存在于8个完整的t单倍型和18个野生型小鼠近交系的缺席。部分f单倍型包含t-特异性片段的子集，并且每种单倍型可以根据其包含的t-特异性片段进行分类。这是第一个分子证据，独立的部分t单倍型包含不同长度的t单倍型DNA。重组研究表明，部分t单倍型抑制重组的程度成比例的t单倍型DNA，它们包含。部分t单倍型的分子分析表明，t特异性片段映射到并因此定义了t复合体的不同区域。由t特异性限制性片段确定的t单倍型DNA的某些区域可以与参与控制传递比畸变的基因座相关。
Four genomic clones obtained from microdissected fragments of the proximal portion of mouse chromosome 17 have been used to identify a series of t-haplotype-specific restriction fragments. Their specificity is defined by presence in eight complete t haplotypes and absence from 18 inbred strains of wild-type mice. Partial f haplotypes contain subsets of the t-specific fragments, and each can be classified according to the t-specific fragments it contains. This is the first molecular evidence that independent partial t haplotypes contain different lengths of t haplotype DNA. Recombination studies indicate that partial t haplotypes suppress recombination in proportion to the extent of t haplotype DNA they contain. Molecular analysis of partial t haplotyes shows that the t-specific fragments map to and thus define different regions of the t complex. Certain regions of t haplotype DNA defined by t-specific restriction fragments can be correlated with loci involved in the control of transmission ratio distortion.