[Integration of DNA clones related to important economic traits of Brassica napus onto Arabidopsis genetic map].
[Integration of DNA clones related to important economic traits of Brassica napus onto Arabidopsis genetic map].
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发表时间：
2002
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通讯作者：
Li-Xia Wang;Jianwei Zhao;Fang-Sen Xu;Ren-Hu Liu;J. Meng
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作者：
Li-Xia Wang;Jianwei Zhao;Fang-Sen Xu;Ren-Hu Liu;J. Meng
拟南芥与芸苔属油料作物是十字花科芸苔科中亲缘关系较近的物种，具有高度同源性。利用拟南芥EST无性系和油菜籽DNA无性系的探针对甘蓝型油菜的一系列重要农艺性状进行了定位，并对25个无性系分别与CMS恢复基因、硼效率基因（BE1）、抗核菌qtl和油菜种间生物量杂种优势性状密切相关进行了DNA片段测序。通过检索相关数据库，在拟南芥基因组中发现高度同源的序列。根据同源序列在拟南芥染色体上的位置，将25个克隆整合到拟南芥遗传图谱中。在拟南芥1号染色体短臂上，将甘蓝型油菜BE1侧的两个标记整合到一个狭窄的区域。其中一个来自该区域的EST克隆pA24被用作定位甘蓝型油菜的探针，并被整合到BE1的两个侧翼标记之间的位置。这一事件增加了人们通过拟南芥和目标芸苔作物之间的相互比较作图来绘制栽培芸苔物种重要特征的信心。
Arbidopsis thaliana and Brassica oilcrops are closely related species in Brassicae, Cruciferae, and show highly homologous genomes. After a serial important agronomic characteristics of Brassica napus being mapped with probes from EST clones of Arabidopsis and DNA clones of rapeseed, DNA segment sequencing was performed with 25 clones which were found to be closely linked to the CMS restore gene, the boron efficiency gene (BE1), the Sclerotinia resistant QTLs and the characteristic of interspecific biomass heterosis in rapeseed respectively. Highly homologous sequences were found in Arabidopsis genome by searching the relative databases. According to the position of homologous sequences on the chromosomes of Arabidopsis, the 25 clones were integrated into the genetic map of Arabidopsis. Two markers flanking BE1 in B. napus were integrated into a narrow region at the short arm of chromosome 1 of Arabidopsis. One of EST clones, pA24, from this region was used as a probe to map B. napus and consequently been integrated in a position between the two flanking markers of BE1. This event increased the confident for one to map the important characteristics of cultured Brassica species via reciprocal comparative mapping between Arabidopsis and target Brassica crops.