LOCALIZATION OF LEUKOCYTE SUBSETS IN THE RAT OVARY DURING THE PERIOVULATORY PERIOD
LOCALIZATION OF LEUKOCYTE SUBSETS IN THE RAT OVARY DURING THE PERIOVULATORY PERIOD
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DOI：
10.1095/biolreprod48.2.277
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发表时间：
1993-02-01
影响因子：
3.6
通讯作者：
ROBERTSON, SA
中科院分区：
文献类型：
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作者：
BRANNSTROM, M;MAYRHOFER, G;ROBERTSON, SA
排卵过程被比作炎症，因为几种经典的炎症介质似乎参与了这一过程。炎症反应的一个组成部分是白细胞迁移到炎症部位，随后这些细胞被激活。我们最近报道，在体外向大鼠卵巢中灌流白细胞可以增加促黄体生成素诱导的排卵次数，这表明白细胞在排卵中起着积极的作用。在本研究中，我们用免疫组织化学方法研究了未处理的未成熟大鼠和注射hCG前、排卵时和排卵后33-36小时处死的未成熟大鼠卵巢中巨噬细胞、T淋巴细胞和粒细胞的分布。用单抗ED1和ED2鉴定的巨噬细胞是主要的白细胞群，主要发现于血管周围的髓质区。在发育、性成熟和排卵后，该区域的细胞密度不断增加。排卵前卵泡的膜层也有巨噬细胞存在，排卵卵泡(hCG后12h)膜层巨噬细胞的数量是排卵前(hCG前)的5倍。两个区域的部分巨噬细胞表达主要组织相容性复合体(MHC)II类抗原(OX6+)，这些细胞主要存在于髓质区，在围排卵期密度没有明显变化。中性粒细胞在髓质区的白细胞总数中所占比例较小，但在膜层中含量较高。与排卵前卵泡相比，排卵期中性粒细胞密度在髓质区增加了3倍，在膜区增加了8倍。T淋巴细胞(OX52+)均匀分布于髓质和皮质间质，密度较低。大多数T淋巴细胞表达CD8抗原(OX8+)，因此属于MHC I类限制性表型。膜层内可见少量T淋巴细胞。综上所述，排卵时卵巢中存在巨噬细胞、中性粒细胞和T淋巴细胞。随着排卵期的进展，髓质区和膜层中巨噬细胞和中性粒细胞的数量有选择性地增加，提示这些细胞可能积极参与排卵时的组织重塑。
The ovulatory process has been compared with inflammation because several classical inflammatory mediators appear to participate in this process. One component of the inflammatory reaction is the migration of leukocytes to the site of inflammation and the subsequent activation of these cells. We have reported recently that perfusion of leukocytes into the rat ovary in vitro enhances the number of LH-induced ovulations, which suggests an active role of leukocytes in ovulation. In the present study we characterize immunohistochemically the distribution of macrophages, T lymphocytes, and granulocytes in the ovaries of untreated immature rats and of eCG-hCG-primed rats killed prior to hCG injection, at ovulation, and at 33-36 h post-ovulation. Macrophages, identified with monoclonal antibodies ED1 and ED2, were the major leukocyte population and were found primarily in the medullary region surrounding the blood vessels. The density of the cells in this region increased continuously during development to sexual maturity and until after ovulation. Macrophages were also present in the thecal layer of the preovulatory follicles, and the numbers of these cells increased about 5-fold in this area in ovulating follicles (12 h after hCG) compared to preovulatory follicles (before hCG). A portion of macrophages in both areas expressed major histocompatibility complex (MHC) class II antigens (OX6+); these cells were present mostly in the medullary region, with no apparent change in density during the periovulatory period. Neutrophilic granulocytes comprised a lesser proportion of the total leukocyte population in the medullary region but were abundant in the thecal layer. The density of neutrophils increased 3-fold in the medullary region and 8-fold in the thecal region in ovulatory compared to preovulatory follicles. T lymphocytes (OX52+) were evenly distributed at relatively low density in the medulla and the stroma of the cortex. Most T lymphocytes expressed the CD8 antigen (OX8+) and hence were of the MHC class I-restricted phenotype. Few T lymphocytes were present in the thecal layer. In summary, macrophages, neutrophilic granulocytes, and T lymphocytes are present in the ovary at ovulation. There is a selective increase in the numbers of macrophages and neutrophilic granulocytes in the medullary region and in the thecal layer as the ovulatory period progresses, indicating that these cells may actively be involved in the tissue remodeling occuring at ovulation.