Performance of a haloalkaliphilic bioreactor and bacterial community shifts under different COD/SO42- ratios and hydraulic retention times
Performance of a haloalkaliphilic bioreactor and bacterial community shifts under different COD/SO42- ratios and hydraulic retention times
复制标题
不同 COD/SO42- 比率和水力保留时间下卤碱生物反应器的性能和细菌群落变化
DOI：
10.1016/j.jhazmat.2014.03.061
复制
发表时间：
2014
影响因子：
13.6
通讯作者：
Xing Jian-Min
中科院分区：
文献类型：
--
作者：
Zhou Jie-Min;Song Zi-Yu;Yan Dao-Jiang;Liu Yi-Lan;Yang Mao-Hua;Cao Hong-Bin;Xing Jian-Min
烟气中的二氧化硫经碱性溶液吸收后转化为硫酸盐。嗜卤碱微生物已用于还原硫酸盐以减少费用并避免硫化物抑制。在模型实验中研究了不同COD/SO42−比率和水力停留时间（HRT）对硫酸盐去除效率和细菌群落的影响。乙醇作为电子供体表现出比乳酸更好的性能。最佳COD/SO42−比和HRT分别为4.0和18 h，硫酸盐去除效率和速率分别为97.8±1.11%和6.26±0.0710 g/L·d。硫化物浓度达到 1603 ± 3.38 mg/L。基于 16S rDNA 的变性梯度凝胶电泳分析，主要的硫酸盐还原菌 (SRB) 是 Desulfonatronovibriosp.，仅在使用乙醇作为电子供体的 COD/SO42− 比率为 4.0 时才检测到。不同的HRT对该物种对应的条带没有显着影响。 PCR结果表明，产甲烷古菌（MPA）来自乙酰分解产甲烷科甲烷八叠球菌科。实时定量 PCR 未显示 SRB 和 MPA 之间存在任何显着竞争。这项研究的结果表明，硫酸盐还原、硝酸盐还原和硫化物氧化可能发生在同一生物反应器中。
Sulfur dioxide from flue gas was converted into sulfate after the absorption of alkaline solutions. Haloalkaliphilic microorganisms have been used in reducing sulfate to decrease expenses and avoid sulfide inhibition. The effects of different COD/SO42−ratios and hydraulic retention times (HRTs) on the sulfate removal efficiency and bacterial community were investigated in model experiments. Ethanol showed better performance as an electron donor than lactate. The optimum COD/SO42−ratio and HRT were 4.0 and 18 h, respectively, with respective sulfate removal efficiency and rate of 97.8 ± 1.11% and 6.26 ± 0.0710 g/L d. Sulfide concentrations reached 1603 ± 3.38 mg/L. Based on denaturing gradient gel electrophoresis analysis of 16S rDNA, the major sulfate-reducing bacterium (SRB) wasDesulfonatronovibriosp., which was only detected at a COD/SO42−ratio of 4.0 using ethanol as an electron donor. Different HRTs had no significant effect on the band corresponding to this species. PCR results show that methane-producing archaea (MPA) were from the acetoclastic methanogenic familyMethanosarcinaceae. Quantitative real-time PCR did not demonstrate any significant competition between SRB and MPA. The findings of this study indicate that sulfate reduction, nitrate reduction, and sulfide oxidization may occur in the same bioreactor.