Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.
Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.
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激活转录所需的因子 (TAF) 与 TATA 盒结合蛋白保守核心结构域相互作用。
DOI：
10.1101/gad.7.2.180
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发表时间：
1993
影响因子：
10.5
通讯作者：
Berk,AJ
中科院分区：
文献类型：
--
作者：
Zhou,Q;Boyer,TG;Berk,AJ
TFIID是一种多亚基蛋白，含有RNA聚合酶II激活转录所需的TATA盒结合多肽（TBP）和相关因子（TFIID-TAF）。来自不同真核生物的TBP含有高度保守的羧基端结构域和非常不同的氨基端结构域。早期的研究提出，后生动物TBPs的氨基末端结构域是激活转录所必需的。然而，我们报告说，一个人的TFIID复合物含有一个氨基末端截短的TBP包含所有主要的TFIID-TAFs和支持在体外转录刺激不同类别的激活结构域和TATA少启动子。蛋白质印迹实验揭示了TBP的保守结构域和两个最大的TAF之间的直接相互作用。结果表明TFIID-TAFs与TBP相互作用的模型。
TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.