HIGHER-ORDER SELF-ASSEMBLY OF VESICLES BY SITE-SPECIFIC BINDING
HIGHER-ORDER SELF-ASSEMBLY OF VESICLES BY SITE-SPECIFIC BINDING
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DOI：
10.1126/science.8209255
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发表时间：
1994-06-17
期刊：
影响因子：
56.9
通讯作者：
ZASADZINSKI, JA
中科院分区：
文献类型：
--
作者：
CHIRUVOLU, S;WALKER, S;ZASADZINSKI, JA
在非特异性分子间力作用下，脂质分子在溶液中形成球形囊泡，这是一个主要的自组装过程。这些囊泡可以通过位点特异性配体-受体（生物素-链亲和素）偶联以可控和可逆的方式进行二次自组装成更高阶的结构。低温电子显微镜显示，这些结构是由原始的无应力状态的栓系而不是粘附的囊泡组成的。相反，非特异性的，如范德华力聚集的囊泡是变形和应力，产生不稳定的结构。通过位点特异性结合的囊泡结合为生产稳定、可控的微结构生物材料提供了一种实用的机制。
The association of lipid molecules into spherical vesicles in solution as a result of nonspecific intermolecular forces constitutes a primary self-assembly process. Such vesicles can undergo a secondary self-assembly into higher order structures in a controlled and reversible manner by means of site-specific ligand-receptor (biotin-streptavidin) coupling. Cryoelectron microscopy shows these structures to be composed of tethered, rather than adhering, vesicles in their original, unstressed state. In contrast, vesicles aggregated by nonspecific, such as van der Waals, forces are deformed and stressed, producing unstable structures. Vesicle association by site-specific binding provides a practical mechanism for the production of stable, yet controllable, microstructured biomaterials.