Anti-influenza virus effect of aqueous extracts from dandelion.
Anti-influenza virus effect of aqueous extracts from dandelion.
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DOI：
10.1186/1743-422x-8-538
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发表时间：
2011-12-14
期刊：
影响因子：
4.8
通讯作者：
Gao B
中科院分区：
文献类型：
--
作者：
He W;Han H;Wang W;Gao B
人类流感是一种季节性疾病，与显著的发病率和死亡率有关。抗流感中药在抗击病毒大流行方面发挥了重要作用。在中医中，蒲公英是许多治疗药物中常用的成分，无论是单独使用还是与其他天然物质联合使用。有证据表明，蒲公英与多种药理活性有关。在这项研究中，我们评价了蒲公英水提物的抗流感病毒活性，并对A型流感病毒、人A/PR/8/34和WSN(H1N1)进行了体外抗病毒活性测试。结果0.625-5 mg/ml的蒲公英提取物对犬肾细胞和人肺腺癌细胞株(A549)的感染有一定的抑制作用，并能抑制病毒的聚合酶活性和病毒核蛋白水平。在有效剂量下，植物提取物对细胞活力、新陈代谢或增殖没有明显的负面影响。这一结果与另一个优势是一致的，即几个世纪以来，这种植物在传统医学中的效用没有任何报道的并发症。蒲公英提取物的抗病毒活性表明，这些提取物的一个或多个成分具有抗流感病毒的特性。蒲公英抑制MDCK或A549细胞病毒生长的机制包括抑制病毒复制。
Human influenza is a seasonal disease associated with significant morbidity and mortality. Anti-flu Traditional Chinese Medicine (TCM) has played a significant role in fighting the virus pandemic. In TCM, dandelion is a commonly used ingredient in many therapeutic remedies, either alone or in conjunction with other natural substances. Evidence suggests that dandelion is associated with a variety of pharmacological activities. In this study, we evaluated anti-influenza virus activity of an aqueous extract from dandelion, which was tested for in vitro antiviral activity against influenza virus type A, human A/PR/8/34 and WSN (H1N1). Results obstained using antiviral assays, minigenome assay and real-time reverse transcription-PCR analysis showed that 0.625-5 mg/ml of dandelion extracts inhibited infections in Madin-Darby canine kidney (MDCK) cells or Human lung adenocarcinoma cell line (A549) of PR8 or WSN viruses, as well as inhibited polymerase activity and reduced virus nucleoprotein (NP) RNA level. The plant extract did not exhibit any apparent negative effects on cell viability, metabolism or proliferation at the effective dose. This result is consistent with the added advantage of lacking any reported complications of the plant's utility in traditional medicine over several centuries. The antiviral activity of dandelion extracts indicates that a component or components of these extracts possess anti-influenza virus properties. Mechanisms of reduction of viral growth in MDCK or A549 cells by dandelion involve inhibition on virus replication.