Deep Sequencing Analysis of microRNAs in Bovine Sperm
Deep Sequencing Analysis of microRNAs in Bovine Sperm
复制标题
牛精子中 microRNA 的深度测序分析
DOI：
10.1002/mrd.22426
复制
发表时间：
2014-11-01
影响因子：
2.5
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Du, Yue;Wang, Xiangguo;Zhang, Yong
MicroRNAs(MiRNAs)是一种小的非编码RNA，参与许多生理途径的调节，但精子传递的miRNAs在受精和胚胎发育中的作用仍存在争议。利用Illumina高通量测序技术构建了牛精子miRNAs文库，并对目的基因进行了预测和通路分析。对哺乳动物精子中的miRNAs进行了系统的研究，并建立了从附睾尾部活检组织中提取RNA的方法。使用BLAST将长度为18-26个核苷酸的独特序列映射到miRBase 20.0中的特定前体。总共鉴定了951个已知的miRNA和8个新的、高表达的miRNA候选基因。寻找内源性精子miRNAs将为胚胎发育和精子表观遗传编程的功能和分子机制研究提供一个初步的数据库。摩尔。是Reprod。戴夫。2014年，81：1042-1052。(C)2014年威利期刊公司。
microRNAs (miRNAs) are small non-coding RNAs that participates in the regulation of many physiological pathways, but a role for spermatozoon-delivered miRNAs in fertilization and embryonic development remains controversial. A library of miRNAs in bovine sperm was constructed using Illumina high-throughput sequencing technology, along with the predication and the pathway analysis of target genes. miRNAs in mammalian spermatozoon were systematically investigated, and a protocol for RNA isolation from the cauda region of an epididymal biopsy was established. Unique sequences that were 18-26 nucleotides in length were mapped to specific precursors in miRBase 20.0 using BLAST. A total of 951 known miRNAs and 8 novel, highly expressed miRNA candidates were identified. The search for endogenous sperm miRNAs will contribute to a preliminary database for functional and molecular mechanistic studies in embryonic development and sperm epigenetic programming. Mol. Reprod. Dev. 81: 1042-1052, 2014. (c) 2014 Wiley Periodicals, Inc.