The Effects of Flavomycin and Colistin Sulfate Pre-Treatment on Ileal Bacterial Community Composition, the Response to Salmonella typhimurium and Host Gene Expression in Broiler Chickens
The Effects of Flavomycin and Colistin Sulfate Pre-Treatment on Ileal Bacterial Community Composition, the Response to Salmonella typhimurium and Host Gene Expression in Broiler Chickens
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黄霉素和硫酸粘菌素预处理对肉鸡回肠细菌群落组成、鼠伤寒沙门氏菌反应及宿主基因表达的影响
DOI：
10.3390/microorganisms7110574
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发表时间：
2019-11-01
期刊：
影响因子：
4.5
通讯作者：
Zhang, Bingkun
中科院分区：
文献类型：
--
作者：
He, Yang;Yang, Yanyan;Zhang, Bingkun
细菌群落的组成影响肉鸡肠道健康和生长性能。本研究旨在探讨黄霉素和硫酸粘杆菌素对鼠伤寒沙门氏菌感染的抵抗力、回肠细菌和肠道健康的影响。将396只1日龄肉仔鸡随机分为6组。两组分别饲喂一种饲料-对照饲料（CON）、10 mg/kg饲料的黄霉素（AntiG+）和40 mg/kg饲料的硫酸粘杆菌素（AntiG−），持续5天。然后，用S.在第8天接种鼠伤寒沙门氏菌，分别命名为CONS、AntiG+S和AntiG−S。结果表明，S.鼠伤寒沙门氏菌显著降低了仔猪的采食量和增重，提高了饲料转化率（P < 0.05）。增加NF-κB和MyD 88基因的炎症表达（p < 0.05），减少紧密连接基因claudin-1、occludin和mucin-2的表达（p < 0.05）。S.鼠伤寒沙门氏菌显著降低了大肠杆菌、Chao 1和Shannon的回肠细菌多样性指数（p < 0.05）。与AntiG+S组相比，AntiG−S组肉鸡增重显著增加（p < 0.05），炎性基因表达显著降低（p < 0.05），肠道对异硫氰酸荧光素的通透性显著降低（p < 0.05）。抗G-S抗体组还能显著提高绵羊和Shannon回肠细菌多样性指数（p < 0.05）。肠道菌群、肠道基因表达与肠道形态之间存在显著相关性（p < 0.05）。这项研究表明，预先构建的AntiG−细菌可以对抗S。通过抑制肠道炎症基因的表达和增加肠道细菌的多样性来抑制鼠伤寒杆菌感染。
The composition of the bacterial community affects the intestinal health and growth performance of broiler chickens. The main purpose of this study was to explore the effects of flavomycin and colistin sulfate on the resistance to Salmonella typhimurium infection, ileal bacteria and intestinal health. In total, 396 1-day-old broiler chickens were randomly divided into six groups. Two groups were fed each one of the diets—the control diet (CON), the flavomycin at 10 mg/kg diet (AntiG+), and the colistin sulfate at 40 mg/kg diet (AntiG−), for 5 days. Then, one of each of the two groups was challenged with S. typhimurium on the 8th day; these were named CONS, AntiG+S and AntiG−S, respectively. The results showed that S. typhimurium significantly reduced the feed intake and body weight gain, and increased the feed conversion ratio (p < 0.05). It also increased the inflammatory expressions of NF-κB and MyD88 genes (p < 0.05); and reduced the expressions of claudin-1, occludin and mucin-2 (p < 0.05) tight junction genes in the intestines. S. typhimurium significantly reduced ileal bacterial diversity indexes of observed-species, chao1 and Shannon (p < 0.05). Compared with AntiG+S group, AntiG−S group increased the body weight gain of broiler chickens (p < 0.05), reduced the expression of inflammatory genes (p < 0.05) and intestinal permeability to fluorescein isothiocyanate (p < 0.05). AntiG-S group also improved the ileal bacterial diversity indexes of observed-species and Shannon (p < 0.05). There were many significant correlations between intestinal bacteria, intestinal gene expressions and intestinal morphology (p < 0.05). This study indicated that pre-constructed AntiG− bacteria could against a S. typhimurium infection by inhibiting the expressions of intestinal inflammation genes and increasing the diversity of intestinal bacteria.