Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs
Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs
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DOI：
10.1182/blood-2006-08-039347
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发表时间：
2007-05-15
期刊：
影响因子：
20.3
通讯作者：
Horwitz, Edwin M.
中科院分区：
文献类型：
--
作者：
Martinez, Caridad;Hofmann, Ted J.;Horwitz, Edwin M.
间充质基质细胞（MSCs）具有巨大的潜力，可用于骨、软骨和其他来源于原始中胚层的组织再生。尽管进行了广泛的研究，但仍然没有一种标记物可以可靠地识别骨髓中的MSC。使用免疫细胞化学和流式细胞术，我们在这里证明，神经节苷脂GD 2表达的MSC无论是新分离的骨髓或扩大在组织培养;这一发现得到了支持的逆转录-聚合酶链反应（RT-PCR）分析显示GD 2合成酶，GD 2生物合成的必需酶的mRNA的表达。GD 2也表达在从脂肪组织分离的MSC上，但不表达在包皮成纤维细胞上。重要的是，MSC是正常骨髓中唯一表达这种标志物的细胞。因此，GD 2似乎是第一个报道的单一表面标记物，独特地区分骨髓间充质干细胞从其他骨髓元素。GD 2可能对研究MSC生物学和制备用于临床应用的MSC有价值。
Mesenchymal stromal cells (MSCs) have enormous potential for the regeneration of bone, cartilage, and other tissues derived from primitive mesoderm. Despite extensive research, there is still no single marker that reliably identifies MSCs within the bone marrow. Using immunocytochemistry and flow cytometry, we demonstrate here that the neural ganglioside GD2 is expressed by MSCs either newly isolated from bone marrow or expanded in tissue culture; this finding was supported by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showing expression of the mRNA for GD2 synthase, an essential enzyme for GD2 biosynthesis. GD2 was also expressed on MSCs isolated from adipose tissue, but not on foreskin fibroblasts. Importantly, MSCs were the only cells within normal marrow that expressed this marker. Thus, GD2 appears to be the first reported single surface marker that uniquely distinguishes MSCs from other marrow elements. GD2 may prove valuable to study MSC biology and for the preparation of MSCs for clinical applications.