Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
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DOI：
10.1038/ncomms4831
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发表时间：
2014-05-01
影响因子：
16.6
通讯作者：
Duchateau, Philippe
中科院分区：
文献类型：
--
作者：
Daboussi, Fayza;Leduc, Sophie;Duchateau, Philippe
硅藻是一类主要的光合作用微藻，具有很高的生物技术潜力，但由于现有遗传工具的匮乏，这一潜力尚未得到充分开发。在这里，我们展示了海洋硅藻三角褐指藻基因组的目标和稳定的修改，使用巨核酸酶和故事核酸酶。当编码核酸酶的构建体与可选择的标记共转化时，很容易获得高频率的基因组修改，分别有56%和27%的菌落表现出靶向突变或靶向基因插入。通过破坏UDP-葡萄糖焦磷酸化酶基因产生了一种增强的产脂菌株(三酰甘油积累增加了45倍)，这证明了基因组工程利用硅藻生产生物燃料的力量。
Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.