The assessment of locomotor function in spinal cord injured rats: The importance of objective analysis of coordination
The assessment of locomotor function in spinal cord injured rats: The importance of objective analysis of coordination
复制标题
DOI：
10.1089/neu.2005.22.214
复制
发表时间：
2005-02-01
影响因子：
4.2
通讯作者：
Joosten, EAJ
中科院分区：
文献类型：
--
作者：
Koopmans, GC;Deumens, R;Joosten, EAJ
Basso，Beattie and Bresna han(BBB)运动评定量表是目前应用最广泛的旷场测试，已被公认为一种评估大鼠脊髓挫伤后运动功能的有效方法。BBB运动评定量表的一个限制是对前肢(FL)-后肢(HL)协调的正确评估。这一限制可能会对运动功能的最终评估产生重大影响。在这项研究中，我们展示了一种基于规则指数(RI)的客观方法来评估协调性，该指数是通过使用T型台方法实现的。RI对协调程度进行评级，其结果是正常步长序列模式的数量乘以4，再除以爪子放置的总量。使用RI可以客观地分析单人行道交叉路口的协调性。将基于T台步的协调性整合到BBB量表中，表明对协调性的客观分析导致了对运动功能的可靠和更敏感的评估。这一新方法已被成功地应用于脊髓损伤(SCI)后强化住房对功能恢复的积极作用的测定。
The Basso, Beattie and Bresnahan (BBB) locomotor rating scale is the most widely used open field test and has been accepted as a valid way to assess locomotor function after spinal cord contusion injury in the rat. A limitation within the BBB locomotor rating scale is the correct assessment of forelimb (FL)-hindlimb (HL) coordination. This limitation can have major implications for the final assessment of locomotor function. In the present study, we show an objective method to assess coordination based on the regularity index (RI), achieved through the use of the CatWalk method. The RI grades the degree of coordination as the result of the number of normal step sequence patterns multiplied by four and divided by the total amount of paw placements. Using the RI, single walkway crossings can be objectively analyzed on coordination. Integration of the CatWalk based coordination into the BBB scale indicates that objective analysis of coordination results in reliable and more sensitive assessment of locomotor function. This new method has been tested successfully in determination of positive effects of enriched housing on functional recovery after spinal cord injury (SCI).