Amino acid supplementation confers protection to red blood cells prior to Plasmodium falciparum bystander stress.
Amino acid supplementation confers protection to red blood cells prior to Plasmodium falciparum bystander stress.
复制标题
补充氨基酸可以在恶性疟原虫旁观者应激之前为红细胞提供保护。
DOI：
10.1101/2023.05.16.540951
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Cordy,ReginaJoice
中科院分区：
文献类型：
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作者：
Binns,HeatherColvin;Alipour,Elmira;Nahid,DinahS;Whitesides,JohnF;Cox,AndersonO'Brien;Furdui,CristinaM;Marrs,GlenS;Kim-Shapiro,DanielB;Cordy,ReginaJoice
摘要疟疾是一种高度氧化性的寄生虫病，贫血是最常见的临床症状。疟疾贫血发病机制的一个主要因素是旁观者未感染的红细胞（rbc）的破坏。已知急性疟疾患者的血浆中会出现代谢波动，这强调了代谢变化在疾病进展和严重程度中的作用。在这里，我们报道了恶性疟原虫培养的条件培养基诱导未感染的过氧化氢酶耗尽的红细胞氧化应激。作为谷胱甘肽的细胞渗透性前体，我们证明了预暴露于外源性谷氨酰胺、半胱氨酸和甘氨酸氨基酸对红细胞的益处。重要的是，这种预处理本质上可以制备红细胞来减轻氧化应激。
AbstractMalaria is a highly oxidative parasitic disease in which anemia is the most common clinical symptom. A major contributor to the malarial anemia pathogenesis is the destruction of bystander, uninfected red blood cells (RBCs). Metabolic fluctuations are known to occur in the plasma of individuals with acute malaria, emphasizing the role of metabolic changes in disease progression and severity. Here, we report conditioned medium fromPlasmodium falciparumculture induces oxidative stress in uninfected, catalase-depleted RBCs. As cell-permeable precursors to glutathione, we demonstrate the benefit of pre-exposure to exogenous glutamine, cysteine, and glycine amino acids for RBCs. Importantly, this pretreatment intrinsically prepares RBCs to mitigate oxidative stress.