Superoxide dismutase and oxidative stress in Dahl salt-sensitive and -resistant rats
Superoxide dismutase and oxidative stress in Dahl salt-sensitive and -resistant rats
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DOI：
10.1152/ajpregu.00346.2001
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发表时间：
2002-09-01
影响因子：
2.8
通讯作者：
Manning, RD
中科院分区：
文献类型：
--
作者：
Meng, SM;Roberts, LJ;Manning, RD
本实验研究了Dahl盐敏感（S）和盐耐受（R）/Rapp品系大鼠在钠摄入变化过程中氧化应激和肾脏超氧化物歧化酶（SOD）水平的作用及其与肾损伤的关系。高钠（8%）饮食3周后，S组大鼠肾髓质Cu/ZnSOD比R组低56%，MnSOD比R组低81%。经过1，2，和3周的高钠，尿中的F-2-异前列腺素的排泄，氧化应激的指数，是显着更大的S大鼠与R大鼠相比。血浆F-2-异前列烷浓度增加2周S高钠喂养组。3 wk后，Dahl S大鼠高钠摄入时肾皮质和髓质超氧化物产生显著增加，S高钠大鼠的尿蛋白排泄量（肾损伤指标）为273 +/-32mg/d，R高钠大鼠为35 +/-4mg/d（P < 0.05）。总之，盐敏感性高血压的S大鼠伴随着显着减少肾髓质SOD和更大的肾脏氧化应激和肾损伤比R大鼠。
The roles of oxidative stress and renal superoxide dismutase (SOD) levels and their association with renal damage were studied in Dahl salt-sensitive (S) and salt-resistant (R)/Rapp strain rats during changes in Na intake. After 3 wk of a high (8%)-Na diet in S rats, renal medullary Cu/Zn SOD was 56% lower and Mn SOD was 81% lower than in R high Na-fed rats. After 1, 2, and 3 wk of high Na, urinary excretion of F-2-isoprostanes, an index of oxidative stress, was significantly greater in S rats compared with R rats. Plasma F-2-isoprostane concentration increased in the 2-wk S high Na-fed group. After 3 wk, renal cortical and medullary superoxide production was significantly increased in Dahl S rats on high Na intake, and urinary protein excretion, an index of renal damage, was 273 +/- 32 mg/d in S high Na-fed rats and 35 +/- 4 mg/d in R high Na-fed rats (P < 0.05). In conclusion, salt-sensitive hypertension in the S rat is accompanied by marked decreases in renal medullary SOD and greater renal oxidative stress and renal damage than in R rats.