Localization of pituitary adenylate cyclase-activating polypeptide and its type I receptors in the rat ovary: immunohistochemistry and in situ hybridization.
Localization of pituitary adenylate cyclase-activating polypeptide and its type I receptors in the rat ovary: immunohistochemistry and in situ hybridization.
复制标题
垂体腺苷酸环化酶激活多肽及其 I 型受体在大鼠卵巢中的定位：免疫组织化学和原位杂交。
DOI：
10.1111/j.1749-6632.1996.tb17540.x
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发表时间：
1996
影响因子：
5.2
通讯作者：
Arimura,A
中科院分区：
文献类型：
--
作者：
Shioda,S;Nakai,Y;Nakajo,S;Nakaya,K;Arimura,A
PACAP是一种新的神经肽，最初从绵羊下丘脑中分离出来，其基础是它能刺激培养的垂体细胞产生cAMP。PACAP有两种形式，分别有38个氨基酸残基(PACAP-38)和27个氨基酸残基(PACAP-27)，由175个氨基酸组成。已有大量使用人工合成的PACAP-38和PACAP-27的研究报道，PACAP的序列表明它是促胰液素/胰高血糖素/血管活性肠肽(VIP)家族的一个新成员，与VIP有最大的相似性。放射免疫分析表明，PACAP-38存在于中枢神经系统的各个组织，包括下丘脑、大脑皮层、海马体和垂体后叶，也存在于许多外周组织，包括胃肠道、肺、肾上腺、睾丸和卵巢。2以前的研究已经确定了PACAP和VIP的结合部位。最近的几项研究表明，VIP刺激多种卵巢功能，包括黄体酮和雌激素的腺体细胞产生，以及雄激素的膜细胞产生。
PACAP is a novel neuropeptide that was originally isolated from ovine hypothalamus on the basis of its ability to stimulate the production of CAMP in cultured pituitary cells.'Two forms, with 38 (PACAP-38) and 27 (PACAP-27) amino acid residues, respectively, are derived from a precursor of 175 amino acids. Numerous studies using synthetic PACAP-38 and PACAP-27 have been reported and the sequence of PACAP suggests that it is a new member of the secretin/glucagon/vasoactive intestinal polypeptide (VIP) family, with greatest similarity with VIP. Radioimmunoassays revealed the presence of PACAP-38 in tissues throughout the central nervous system, including the hypothalamus, cerebral cortex, hippocampus, and posterior pituitary, and also in many peripheral tissues, including the gastrointestinal tract, lung, adrenal, testis, and ovary. 2 Previous studies have identified both PACAP-and VIP-binding sites in the Several recent studies have shown that VIP stimulates a variety of ovarian functions, including ov~ lation,~ production by glanulosa cells of progesterone and estr0gen, 6.~.'and production by theca cells of androgen.','Zhong