Interface engineering of microelectrodes toward ultrasensitive monitoring of β-amyloid peptides in cerebrospinal fluid in Alzheimer's disease
Interface engineering of microelectrodes toward ultrasensitive monitoring of β-amyloid peptides in cerebrospinal fluid in Alzheimer's disease
复制标题
微电极界面工程对阿尔茨海默氏病脑脊液中β-淀粉样肽的超灵敏监测
DOI：
10.1039/c9an02285f
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发表时间：
2020-03-21
期刊：
影响因子：
4.2
通讯作者：
Shi, Guoyue
中科院分区：
文献类型：
--
作者：
Ding, Shushu;Xu, Yunxia;Shi, Guoyue
单体β-淀粉样肽的超灵敏检测对于研究阿尔茨海默病（AD）的病理过程具有重要意义。在这篇文章中，我们开发了一种新的电化学生物传感器，通过简易工程的金微电极接口，用于敏感和选择性监测脑脊液（CSF）中的β-淀粉样肽（A β）单体。通过特异性的Cu 2 +-A beta-hemin配位，A beta-hemin引导Cu 2 +-PEI/AuNPs-hemin纳米探针在微电极界面组装成网络聚集体，促进了A beta-hemin单体在微电极上的富集。此外，AuNP聚集体促进银纳米颗粒的沉积，其用于A β单体的电化学溶出分析。所提出的方法显示出超灵敏度的A β单体的检测限低至0.2 pM。此外，观察到对A β单体的高选择性。这些卓越的分析性能使得电化学生物传感器可用于评估AD活小鼠CSF中A β单体水平的动态变化，促进对A β单体在脑事件中所起作用的研究。
Ultrasensitive detection of monomeric beta-amyloid peptides is of fundamental significance for studying the pathological progression of Alzheimer's disease (AD). In this article, by facilely engineering a gold microelectrode interface, we developed a novel electrochemical biosensor for sensitive and selective monitoring of beta-amyloid peptide (A beta) monomers in cerebrospinal fluid (CSF). Through specific Cu2+-A beta-hemin coordination, A beta directed the assembly of Cu2+-PEI/AuNPs-hemin nanoprobes into network aggregates on a microelectrode interface, which promoted the enrichment of A beta monomers on the microelectrode. Furthermore, the AuNP aggregate promotes the deposition of silver nanoparticles, which were utilized for the electrochemical stripping analysis of the A beta monomer. The proposed method displayed ultra-sensitivity for A beta monomers with the detection limit down to 0.2 pM. Besides, high selectivity toward A beta monomers was observed. These remarkable analytical performances render the electrochemical biosensor useful for evaluating the dynamic change of A beta monomer level in CSF of live mice with AD, promoting the investigation of the role that A beta monomers play in brain events.