GENETIC-VARIATION DETECTABLE WITH MOLECULAR MARKERS AMONG UNADAPTED GERM-PLASM RESOURCES OF CULTIVATED PEANUT AND RELATED WILD-SPECIES
GENETIC-VARIATION DETECTABLE WITH MOLECULAR MARKERS AMONG UNADAPTED GERM-PLASM RESOURCES OF CULTIVATED PEANUT AND RELATED WILD-SPECIES
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DOI：
10.1139/g91-156
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发表时间：
1991-12-01
期刊：
影响因子：
3.1
通讯作者：
KOCHERT, G
中科院分区：
文献类型：
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作者：
HALWARD, TM;STALKER, HT;KOCHERT, G
花生种质是由栽培的异源四倍体花生（Arachis hypogaea L.）还有大量的野生物种，几乎都是二倍体。我们以前的工作表明，一个非常低的水平，在美国品种的遗传变异，限制性片段长度多态性（RFLP）分析测定。由于美国栽培品种可能代表一个狭窄的遗传基础，我们扩大了我们的研究，包括来自南美的起源，非洲和中国，其中相当大的形态和生理变异已被报道存在的各个中心不适应的种质系。在评价中包括花生组的野生种，因为它们在通过RFLP测定时显示出高度变异。三种方法用于测定RFLP变异：（i）常规RFLP分析，使用随机基因组克隆从花生和苜蓿（紫花苜蓿）的cDNA克隆;（ii）聚合酶链反应（PCR）扩增的随机引物序列;（iii）四切割分析的PCR扩增片段。在所有的情况下，一个非常低的水平的变异被发现在栽培花生，而丰富的变异存在于野生二倍体物种。本文就花生的进化和对花生育种的意义进行了讨论。
Peanut germ plasm consists of the cultivated allotetraploid species Arachis hypogaea L. and a large number of wild species, which are nearly all diploids. Our previous work indicated a very low level of genetic variability in American cultivars, as assayed by restriction fragment length polymorphism (RFLP) analysis. Since American cultivars might represent a narrow genetic base, we expanded our study to include unadapted germ-plasm lines from the various South American centers of origin, Africa, and China, where considerable morphological and physiological variability has been reported to exist. Wild species of section Arachis were included in the evaluations since they show a high degree of variation when assayed by RFLPs. Three methods were used to assay for RFLP variation: (i) conventional RFLP analysis using random genomic clones from peanut and cDNA clones from peanut and alfalfa (Medicago sativa); (ii) polymerase chain reaction (PCR) amplification of random primer sequences; (iii) four-cutter analysis of PCR-amplified fragments. In all cases a very low level of variability was found in cultivated peanut, while abundant variability was present among wild diploid species. The results are discussed in terms of peanut evolution and significance to peanut breeding.