The Relationship between Cell Number, Division Behavior and Developmental Potential of Cleavage Stage Human Embryos: A Time-Lapse Study.
The Relationship between Cell Number, Division Behavior and Developmental Potential of Cleavage Stage Human Embryos: A Time-Lapse Study.
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卵裂期人类胚胎的细胞数量、分裂行为和发育潜力之间的关系：一项延时研究
DOI：
10.1371/journal.pone.0153697
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Lin G
中科院分区：
文献类型：
--
作者：
Kong X;Yang S;Gong F;Lu C;Zhang S;Lu G;Lin G
第3天卵裂胚胎移植是今天许多辅助生殖技术中心的常规。通常根据细胞数量、细胞对称性和碎片选择胚胎进行转移。许多研究表明，细胞数量与胚胎发育潜力之间存在关系。然而，对胚胎分裂行为及其与胚胎细胞数量和发育潜力的关系的理解有限。本研究采用回顾性和观察性研究方法，通过延时成像研究不同的分裂行为如何影响第3天胚胎的细胞数量和发育潜力。根据第3天的细胞数量，（来自104个IVF/卵胞浆内单精子注射（ICSI）治疗周期，n = 799）分类如下：少于5个细胞（< 5C; n = 111）; 5-6个细胞（5-6C; n = 97）; 7-8个细胞（7- 8 C; n = 442），9-10个细胞（9- 10 C; n = 107）和多于10个细胞（> 10 C; n = 42）。分析5组不同细胞数的3天胚胎的分裂行为、形态动力学参数和囊胚形成率。在<5C和5-6C胚胎中，碎片化（FR;分别为62.2%和30.9%）是导致细胞数量减少的主要原因。大多数7- 8 C胚胎在发育过程中表现出明显的正常行为（NB; 85.7%）。然而，与7- 8 C胚胎相比，9- 10 C和> 10 C胚胎中DC的发生率增加（分别为45.8%、33.3%和11.1%）。在≥5C的胚胎中，FR和DC显著降低发育潜力，而<5C的胚胎无论分裂行为如何都表现出很小的潜力。在NB胚胎中，囊胚形成率随细胞数的增加而增加，从7.4%（<5C）增加到89.3%（> 10 C）。在NB胚胎中，细胞周期延长或缩短分别是细胞数量异常低或异常高的主要原因。剔除分裂行为异常的胚胎后，第3天胚胎的发育潜能、着床率和活产率随细胞数的增加而增加。
Day 3 cleavage embryo transfer is routine in many assisted reproductive technology centers today. Embryos are usually selected according to cell number, cell symmetry and fragmentation for transfer. Many studies have showed the relationship between cell number and embryo developmental potential. However, there is limited understanding of embryo division behavior and their association with embryo cell number and developmental potential. A retrospective and observational study was conducted to investigate how different division behaviors affect cell number and developmental potential of day 3 embryos by time-lapse imaging. Based on cell number at day 3, the embryos (from 104 IVF/intracytoplasmic sperm injection (ICSI) treatment cycles, n = 799) were classified as follows: less than 5 cells (< 5C; n = 111); 5–6 cells (5–6C; n = 97); 7–8 cells (7–8C; n = 442), 9–10 cells (9–10C; n = 107) and more than 10 cells (>10C; n = 42). Division behavior, morphokinetic parameters and blastocyst formation rate were analyzed in 5 groups of day 3 embryos with different cell numbers. In <5C and 5–6C embryos, fragmentation (FR; 62.2% and 30.9%, respectively) was the main cause for low cell number. The majority of 7–8C embryos exhibited obvious normal behaviors (NB; 85.7%) during development. However, the incidence of DC in 9–10C and >10C embryos increased compared to 7–8C embryos (45.8%, 33.3% vs. 11.1%, respectively). In ≥5C embryos, FR and DC significantly reduced developmental potential, whereas <5C embryos showed little potential irrespective of division behaviors. In NB embryos, the blastocyst formation rate increased with cell number from 7.4% (<5C) to 89.3% (>10C). In NB embryos, the cell cycle elongation or shortening was the main cause for abnormally low or high cell number, respectively. After excluding embryos with abnormal division behaviors, the developmental potential, implantation rate and live birth rate of day 3 embryos increased with cell number.