Isolation of a major cell surface glycoprotein from fibroblasts.
Isolation of a major cell surface glycoprotein from fibroblasts.
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从成纤维细胞中分离主要细胞表面糖蛋白。
DOI：
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发表时间：
1974
影响因子：
11.1
通讯作者：
J. A. Weston
中科院分区：
文献类型：
--
作者：
K. Yamada;J. A. Weston
从培养的鸡胚和鸡心脏成纤维细胞中分离出部分纯化的细胞表面成分。这种糖蛋白与最近报道的存在于正常细胞表面，在肿瘤转化后缺失的一种蛋白质相似。它是一种主要的细胞表面糖蛋白，由培养成纤维细胞合成，但不是胶原蛋白。它对胰蛋白酶具有明显的敏感性，其从细胞表面的恢复依赖于细胞密度。它被排除在Sephadex G-200之外，但不能通过超离心快速沉淀，表观分子量为220,000。这种细胞表面糖蛋白的分离现在可以提供一种确定其功能的方法。
A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present at the surface of normal cells, and missing after neoplastic transformation. It is a major cell surface glycoprotein that is synthesized by cultured fibroblasts, but is not collagen. It is shown to be markedly trypsin-sensitive, and its recovery from the cell surface is dependent on cell density. It is excluded from Sephadex G-200, but is not rapidly sedimented by ultracentrifugation, and has an apparent molecular weight of 220,000. The isolation of this cell surface glycoprotein may now provide a means of determining its function.