Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells
Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells
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DOI：
10.1002/mabi.200800123
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发表时间：
2008-12-08
影响因子：
4.6
通讯作者：
Mailaender, Volker
中科院分区：
文献类型：
--
作者：
Dausend, Julia;Musyanovych, Anna;Mailaender, Volker
研究了荷电聚苯乙烯纳米颗粒进入HeLa细胞的内吞机制。在存在或不存在已知抑制内吞作用中的各种因子的药物的情况下进行摄取实验。独立的粒子电荷，内吞作用是高度依赖于F-肌动蛋白，酪氨酸特异性蛋白激酶，这表明动力依赖和脂筏依赖的机制。然而，胆固醇消耗并不妨碍颗粒摄取。关于带正电荷的粒子，巨胞饮作用、微管网络和环加氧酶也参与其中。网格蛋白依赖性途径起次要作用。
The endocytotic mechanisms involved in the uptake of charged polystyrene nanoparticles into HeLa cells were investigated. Uptake experiments were done in the presence or absence of drugs known to inhibit various factors in endocytosis. Independent of the particle charge, endocytosis is highly dependent on F-actin, and tyrosine-specific protein kinases, which suggests a dynamin-dependent and lipid raft-dependent mechanism. However, cholesterol depletion did not hinder particle uptake. Regarding positively charged particles, macropinocytosis, the microtubule network, and cyclooxygenases are also involved. The clathrin-dependent pathway plays a minor role.