Development and characterization of monoclonal antibody to T-antigen:: (Galβ1-3GalNAc-α-O)
Development and characterization of monoclonal antibody to T-antigen:: (Galβ1-3GalNAc-α-O)
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DOI：
10.1089/hyb.1998.17.165
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发表时间：
1998-04-01
期刊：
影响因子：
--
通讯作者：
Roy, R
中科院分区：
文献类型：
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作者：
Rittenhouse-Diakun, K;Xia, Z;Roy, R
糖抗原Gal β 1-3GalNAc或T抗原在许多系统中具有生物学重要性。Gal β 1-3GalNAc是一种肿瘤相关的糖类抗原，在生殖中心B细胞和皮质T细胞中暂时表达的抗原，寄生虫相关抗原，精子活力标志物和老化红细胞上的抗原，它可能在正常细胞粘附和肿瘤细胞转移中起作用。我们通过用合成的Gal β 1 - 3GalNAc-BSA缀合物免疫小鼠开发了针对Gal β 1 - 3GalNAc的IgG（3）MAb。使用抑制酶免疫测定与相关的合成制备的糖进行分析，以确定抗体结合中涉及的限制，使用合成糖作为竞争对手使我们能够描绘对该单克隆抗体的结合活性的表位限制，并将使该单克隆抗体在关于该二糖的生物学重要性的研究中使用。
The saccharide antigen, Gal beta 1-3GalNAc or T antigen, is of biologic importance in many systems. It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells, It may play a role in normal cellular adhesion and in tumor cell metastasis, Well characterized monoclonal antibodies (MAb) to Gal beta 1-3GalNAc will be useful for further studies in these areas. We developed an IgG(3) MAb to Gal beta 1-3GalNAc by immunizing the mice with a synthetic Gal beta 1-3GalNAc-BSA conjugate. The MAb was analyzed using inhibition enzyme immunoassays with related synthetically prepared sugars to determine the restrictions involved in the antibody binding, Use of synthetic sugars as competitors enabled us to delineate the epitope restrictions on the binding activity of this monoclonal and will enable use of this MAb in studies concerning the biologic importance of this disaccharide.