Comparison of expression patterns for placenta growth factor, vascular endothelial growth factor (VEGF), VEGF-B and VEGF-C in the human placenta throughout gestation
Comparison of expression patterns for placenta growth factor, vascular endothelial growth factor (VEGF), VEGF-B and VEGF-C in the human placenta throughout gestation
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DOI：
10.1677/joe.0.1590459
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发表时间：
1998-12-01
影响因子：
4
通讯作者：
Charnock-Jones, DS
中科院分区：
文献类型：
--
作者：
Clark, DE;Smith, SK;Charnock-Jones, DS
血管生成和血管转化是胎盘正常发育的重要过程。血管内皮生长因子（VEGF）是一种有效的血管生成生长因子，被认为对胎盘发育很重要。最近，这个家族的几个新成员被描述。在这项研究中，我们使用原位杂交来定位胎盘中哪些细胞表达VEGF、胎盘生长因子（PlGF）、VEGF-B和VEGF-C的mRNA。我们无法在胎盘中找到VEGF-B或VEGF-C的任何信息，这表明仅产生了这种方法无法检测的低水平。编码VEGF的mRNA被发现是由绒毛间充质、蜕膜巨噬细胞和蜕膜腺内的细胞产生的，但与我们以前的研究结果相反，不是由滋养层产生的。编码PlGF的mRNA由绒毛细胞滋养层、合体滋养层和绒毛外滋养层大量产生。因此，编码VEGF和PlGF的mRNA没有共定位，并且似乎在胎盘中不可能有任何显著的VEGF/PlGF异源二聚体产生。
Angiogenesis and vascular transformation are important processes in the normal development of the placenta. Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor and is thought to be important for placental development. Recently several new members of this family have been described. In this study we used in situ hybridisation to localise which cells in the placenta expressed mRNA for VEGF, placenta growth factor (PlGF), VEGF-B and VEGF-C. We were unable to find any message for either VEGF-B or VEGF-C in the placenta, suggesting that only low levels are produced which this method was unable to detect. The mRNA encoding VEGF was found to be produced by cells within the villous mesenchyme, decidual macrophages and decidual glands but, in contrast to our previous findings, not by trophoblast. The mRNA encoding PlGF was produced in large amounts by villous cytotrophoblast, syncytiotrophoblast and extravillous trophoblast. The mRNAs encoding VEGF and PlGF were thus not co-localised and it appears that there is unlikely to be any significant production of VEGF/PlGF heterodimer in the placenta.