Immunohistochemical localization of constitutive and inducible cyclo-oxygenases in rat uterus during the oestrous cycle and pregnancy.
Immunohistochemical localization of constitutive and inducible cyclo-oxygenases in rat uterus during the oestrous cycle and pregnancy.
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发情周期和妊娠期间大鼠子宫中组成型和诱导型环氧合酶的免疫组织化学定位。
DOI：
10.1023/a:1003228427487
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Yallampalli,C
中科院分区：
文献类型：
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作者：
Fang,L;Chatterjee,S;Dong,YL;Gangula,PR;Yallampalli,C
子宫是花生四烯酸代谢通过环氧合酶途径合成类花生酸的丰富来源。环氧合酶有两种同工型，组成型（COX-I）和诱导型（COX-II）。在本研究中，我们已经在大鼠子宫的各种生理状态下的COX-I和COX-II的分布进行了化学作图。从雌性大鼠（a）发情周期的不同阶段，（B）妊娠第1、4、8和18天，（c）自然分娩后和（d）产后采集子宫组织，并固定在布因固定液中。石蜡包埋后，5 μ m厚的切片用ABC技术进行化学染色。在光学显微镜下观察染色的切片显示，在非妊娠大鼠子宫中，COX-1和COX-II在子宫内膜中大量表达，在子宫肌层中观察到最小的染色。染色在上皮细胞中比在基质细胞中更突出。上皮细胞的染色强度在发情前期和发情期最高，在间情期最低。在妊娠大鼠中，虽然COX-Ⅰ和COX-Ⅱ的表达主要定位于子宫内膜，在妊娠第1天的子宫肌层中几乎没有染色，但这两种酶在妊娠第4天的子宫肌层细胞中也很明显。子宫内膜和子宫肌层细胞的染色强度随着妊娠的进展而进一步增加，在自然分娩时达到最大。然而，在产后期间，子宫内膜和子宫肌层中的两种酶的染色强度均降低。因此，我们的研究表明，环加氧酶的表达在不同的子宫细胞与发情周期和妊娠的变化。此外，在妊娠和分娩期间子宫肌层中环加氧酶的表达显著增加，这意味着子宫肌层中的环加氧酶系统可能在妊娠和分娩期间调节子宫收缩力中发挥重要作用。© 1998年查普曼和霍尔
The uterus is a rich source of eicosanoids synthesized from arachidonic acid metabolism through the cyclo-oxygenase pathway. Two isoforms of cyclo-oxygenase, constitutive (COX-I) and inducible (COX-II) enzyme, have been reported. In the present study, we have immunohistochemically mapped the distribution of both COX-I and COX-II during various physiological states of the rat uterus. Uterine tissue was collected from female rats (a) during different stages of the oestrous cycle, (b) on days 1, 4, 8 and 18 of gestation, (c) after spontaneous delivery and (d) post partum, and fixed in Bouin's fixative. After paraffin wax embedding, 5-μm-thick sections were immunohistochemically stained by the ABC technique. Observation of the stained sections under the light microscope revealed that, in non-pregnant rat uterus, both COX-I and COX-II were abundantly expressed in the endometrium, with minimal staining observed in the myometrium. Staining was more prominent in epithelial cells than in stromal cells. The intensity of staining in epithelial cells was highest at pro-oestrus and oestrus and lowest at dioestrus. In pregnant rats, although the expression of both COX-I and COX-II was localized primarily to the endometrium with very little staining in the myometrium on day 1 of gestation, both of these enzymes were also apparent in myometrial cells by day 4 of gestation. The staining intensity of endometrial and myometrial cells increased further with the progression of gestation, being maximal at the time of spontaneous delivery. During the post-partum period, however, the staining intensity for both of the enzymes in endometrium and myometrium was decreased. Thus, our studies show that the expression of cyclo-oxygenases in various uterine cells vary with the oestrous cycle and with pregnancy. Furthermore, prominent increases in the expression of cyclo-oxygenases in the myometrium during pregnancy and parturition imply that the cyclo-oxygenase system in the myometrium may play a major role in modulating uterine contractility during pregnancy and labour. © 1998 Chapman & Hall