Immunohistochemical evaluation of insulin-like growth factor I receptor status in cervical cancer specimens.
Immunohistochemical evaluation of insulin-like growth factor I receptor status in cervical cancer specimens.
复制标题
宫颈癌标本中胰岛素样生长因子 I 受体状态的免疫组织化学评估。
作者：
H. Kuramoto;A. Hongo;Yi;Y. Ojima;K. Nakamura;N. Seki;J. Kodama;Y. Hiramatsu
胰岛素样生长因子I受体（IGF-IR）在许多宫颈癌衍生细胞系中异常过表达。据推测，由于人乳头瘤病毒（HPV）感染导致的p53蛋白水平的降低可能有助于宫颈癌细胞中IGF-IR表达的上调，因为IGF-IR的转录被p53严格下调。为了评估这一事实在临床宫颈癌标本，我们检查IGF-IR的表达水平和激活状态的免疫组化。通过锥切或子宫切除术获得的福尔马林固定和石蜡包埋标本用抗IGF-IR和识别其C末端磷酸化酪氨酸的抗体染色。IGF-IR在宫颈上皮内瘤样病变（CIN）III和浸润性癌中的表达水平显著增高。IGF-IR磷酸化在所有CIN和浸润性癌标本中均被促进，其强度与病变的促进程度相关。有趣的是，IGF-IR过表达在CIN I和II病变的基底层中缺失，而在CIN III和浸润性癌病变中均匀分布。IGF-IR过表达模式可用于CIN病变中HPV感染状态的诊断。
The insulin-like growth factor I receptor (IGF-IR) is exceptionally overexpressed in many cervical-cancer-derived cell lines. It is postulated that a decrease of p53 protein levels due to human papillomavirus (HPV) infection may contribute to the up-regulation of IGF-IR expression in cervical cancer cells because transcription of IGF-IR is strictly down-regulated by p53. To evaluate this fact in clinical cervical cancer specimens, we checked the expression levels and activated status of IGF-IR by immunohistochemistry. Formalin-fixed and paraffin-embedded specimens obtained by conization or hysterectomy were stained with anti-IGF-IR and with an antibody recognizing phosphorylated tyrosine at its c-terminus. The expression levels of IGF-IR were significantly high in cervical intraepithelial neoplasia (CIN) III and invasive cancer specimens. Phosphorylation of IGF-IR was promoted in all CIN and invasive cancer specimens, and its intensity was related to the promotion of lesions. Interestingly, IGF-IR overexpression was missing in the basal layer of CIN I and II lesions, whereas it was evenly distributed in CIN III and invasive cancer lesions. This IGF-IR overexpression pattern may be utilized in the diagnosis of HPV infection status in CIN lesions.
影响因子：
3.3
作者：
M. Stoler;C. R. Rhodes;A. Whitbeck;Steven Wolinsky;L. Chow;T. Broker
通讯作者：
M. Stoler;C. R. Rhodes;A. Whitbeck;Steven Wolinsky;L. Chow;T. Broker
可检测人类癌症中激活的 IGF-I 受体的抗体的表征。
DOI：
10.1006/excr.1999.4562
发表时间：
1999
期刊：
Experimental cell research.
影响因子：
--
作者：
Rubini,M;D'Ambrosio,C;Carturan,S;Yumet,G;Catalano,E;Shan,S;Huang,Z;Criscuolo,M;Pifferi,M;Baserga,R
通讯作者：
Baserga,R
影响因子：
3.7
作者：
Jones, DL;Thompson, DA;Münger, K
通讯作者：
Münger, K