A new photobioreactor concept enabling the production of desiccation induced biotechnological products using terrestrial cyanobacteria
A new photobioreactor concept enabling the production of desiccation induced biotechnological products using terrestrial cyanobacteria
复制标题
DOI：
10.1016/j.jbiotec.2014.10.002
复制
发表时间：
2014-12-20
影响因子：
4.1
通讯作者：
Ulber, R.
中科院分区：
文献类型：
--
作者：
Kuhne, S.;Strieth, D.;Ulber, R.
蓝细菌为制药应用的生物技术产品的生产提供了巨大的潜力。然而，这些生物只能使用光生物反应器（PBR）有效地培养。然而，在淹没条件下，陆地蓝藻大多以次优的方式生长，这使得这种培养技术不经济，因此陆地蓝藻没有吸引力。因此，一种新型的浮出式光生物反应器（ePBR）被开发出来，它可以为这些生物提供自然条件。概念验证以及第一效率测试进行使用陆地蓝藻Trichocoleus sociatus作为模式生物。T.在淹没系统中，sociatus（0.014 +/- 0.001 h（-1））可以增加35%。此外，现在可以控制干燥相关的产物形成和相关的代谢过程。这显示了胞外聚合物（EPS）的产生。在这种情况下，0.068 +/- 0.006 g EPS/g DW的产量可以增加7倍以上。(C)2014爱思唯尔有限公司版权所有。
Cyanobacteria offer great potential for the production of biotechnological products for pharmaceutical applications. However, these organisms can only be cultivated efficiently using photobioreactors (PBR). Under submerged conditions though, terrestrial cyanobacteria mostly grow in a suboptimal way, which makes this cultivation-technique uneconomic and thus terrestrial cyanobacteria unattractive. Therefore, a novel emersed photobioreactor (ePBR) has been developed, which can provide the natural conditions for these organisms. Proof of concept as well as first efficiency tests are conducted using the terrestrial cyanobacteria Trichocoleus sociatus as a model organism. The initial maximum growth rate of T. sociatus (0.014 +/- 0.001 h(-1)) in submerged systems could be increased by 35%. Furthermore, it is now possible to control desiccation-correlated product formation and related metabolic processes. This is shown for the production of extracellular polymeric substances (EPS). In this case the yield of 0.068 +/- 0.006 g of EPS/g DW could be increased by more than seven times. (C) 2014 Elsevier B.V. All rights reserved.