The Amphibian Antimicrobial Peptide Temporin B Inhibits In Vitro Herpes Simplex Virus 1 Infection.
The Amphibian Antimicrobial Peptide Temporin B Inhibits In Vitro Herpes Simplex Virus 1 Infection.
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DOI：
10.1128/aac.02367-17
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发表时间：
2018-05
影响因子：
4.9
通讯作者：
Nencioni L
中科院分区：
文献类型：
--
作者：
Marcocci ME;Amatore D;Villa S;Casciaro B;Aimola P;Franci G;Grieco P;Galdiero M;Palamara AT;Mangoni ML;Nencioni L
单纯疱疹病毒 1 (HSV-1) 在人群中广泛传播，大多数情况下其感染是无症状的。目前可用的抗HSV-1药物是阿昔洛韦及其衍生物，但长期使用这些药物治疗可导致耐药性。因此，新型抗疱疹化合物的发现值得付出更多努力。天然存在的抗菌肽 (AMP) 是一类具有潜在抗病毒特性的有趣分子。据我们所知，这项研究首次证明了 temporin B (TB)（一种短膜活性两栖动物 AMP）的体外抗 HSV-1 活性。特别是，当 HSV-1 与 20 μg/ml TB 预孵育时，观察到显着的抗病毒活性（病毒滴度降低 5 个对数）。正如透射电子显微镜所证明的，这种效应是由于病毒包膜被破坏所致。此外，结核病部分影响了HSV-1生命周期的不同阶段，包括病毒附着和进入宿主细胞，以及随后的感染后阶段。此外，其对人类上皮细胞的功效得到证实，表明结核病是预防和/或治疗 HSV-1 感染的新方法。
The herpes simplex virus 1 (HSV-1) is widespread in the population, and in most cases its infection is asymptomatic. The currently available anti-HSV-1 drugs are acyclovir and its derivatives, although long-term therapy with these agents can lead to drug resistance. Thus, the discovery of novel antiherpetic compounds deserves additional effort. Naturally occurring antimicrobial peptides (AMPs) represent an interesting class of molecules with potential antiviral properties. To the best of our knowledge, this study is the first demonstration of the in vitro anti-HSV-1 activity of temporin B (TB), a short membrane-active amphibian AMP. In particular, when HSV-1 was preincubated with 20 μg/ml TB, significant antiviral activity was observed (a 5-log reduction of the virus titer). Such an effect was due to the disruption of the viral envelope, as demonstrated by transmission electron microscopy. Moreover, TB partially affected different stages of the HSV-1 life cycle, including the attachment and the entry of the virus into the host cell, as well as the subsequent postinfection phase. Furthermore, its efficacy was confirmed on human epithelial cells, suggesting TB as a novel approach for the prevention and/or treatment of HSV-1 infections.