An Atlas of Brain-Bone Sympathetic Neural Circuits.
An Atlas of Brain-Bone Sympathetic Neural Circuits.
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脑骨交感神经回路图谱。
DOI：
10.1101/2024.02.07.579382
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Zaidi,Mone
中科院分区：
文献类型：
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作者：
Ryu,Vitaly;Gumerova,Anisa;Witztum,Ronit;Korkmaz,Funda;Kannangara,Hasni;Moldavski,Ofer;Barak,Orly;Lizneva,Daria;Goosens,KiA;Stanley,Sarah;Kim,Se-Min;Yuen,Tony;Zaidi,Mone
有明确的证据表明，交感神经系统（SNS）介导骨代谢。组织学研究显示骨膜和骨髓有丰富的SNS神经支配--这些神经由去甲肾上腺素能纤维组成，这些纤维对酪氨酸羟化酶、多巴胺β羟化酶或神经肽Y进行免疫染色。尽管如此，发送传出SNS流出到骨骼的大脑部位尚未被表征。使用伪狂犬病（PRV）病毒transneuronal追踪，我们报告，第一次，中央SNS流出网站，支配骨的识别。我们发现，中央SNS流出到骨起源于87个脑核团，亚核团和6个脑分区，即中脑和脑桥，下丘脑，后脑髓质，前脑，大脑皮质和丘脑的区域。我们还发现，某些网站，如中缝大（RMg）的髓质和中脑导水管周围灰质（PAG），显示更大程度的PRV 152感染，这表明有相当大的网站特定的变化，在中央SNS流出到骨的水平。这一全面的纲要说明了中枢编码和控制的SNS传出信号骨应允许更好地了解骨代谢的神经调节，重要的是和临床相关性，中枢性骨痛的机制。
There is clear evidence that the sympathetic nervous system (SNS) mediates bone metabolism. Histological studies show abundant SNS innervation of the periosteum and bone marrow––these nerves consist of noradrenergic fibers that immunostain for tyrosine hydroxylase, dopamine beta hydroxylase, or neuropeptide Y. Nonetheless, the brain sites that send efferent SNS outflow to bone have not yet been characterized. Using pseudorabies (PRV) viral transneuronal tracing, we report, for the first time, the identification of central SNS outflow sites that innervate bone. We find that the central SNS outflow to bone originates from 87 brain nuclei, sub–nuclei and regions of six brain divisions, namely the midbrain and pons, hypothalamus, hindbrain medulla, forebrain, cerebral cortex, and thalamus. We also find that certain sites, such as the raphe magnus (RMg) of the medulla and periaqueductal gray (PAG) of the midbrain, display greater degrees of PRV152 infection, suggesting that there is considerable site–specific variation in the levels of central SNS outflow to bone. This comprehensive compendium illustrating the central coding and control of SNS efferent signals to bone should allow for a greater understanding of the neural regulation of bone metabolism, and importantly and of clinical relevance, mechanisms for central bone pain.