A high-salt/high fat diet alters circadian locomotor activity and glucocorticoid synthesis in mice
A high-salt/high fat diet alters circadian locomotor activity and glucocorticoid synthesis in mice
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DOI：
10.1371/journal.pone.0233386
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发表时间：
2020-05
期刊：
影响因子：
3.7
通讯作者：
Y. Yokoyama;Takahiro J. Nakamura;Karen Yoshimoto;Honoka Ijyuin;Naoyuki Tachikawa;Haruka Oda;Rena Shiraishi;Kaori Shinohara;Kayo Kumadaki;S. Honda;Anna Nakamura;Naho Kitamura;Kazuo Tsubota;Mitsuhiro Watanabe
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文献类型：
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作者：
Y. Yokoyama;Takahiro J. Nakamura;Karen Yoshimoto;Honoka Ijyuin;Naoyuki Tachikawa;Haruka Oda;Rena Shiraishi;Kaori Shinohara;Kayo Kumadaki;S. Honda;Anna Nakamura;Naho Kitamura;Kazuo Tsubota;Mitsuhiro Watanabe
盐是一种必需的营养素;然而，过量的盐摄入量是全球一个突出的公共卫生问题。各种生理功能与昼夜节律相关，昼夜节律的破坏是心血管疾病、癌症和免疫疾病的突出风险因素。某些营养素是外周生物钟的重要调节剂。然而，高脂高盐（HFS）饮食在调节昼夜节律基因表达中的作用尚不清楚。本研究的目的是探讨HFS饮食对小鼠运动活动节律、盲肠糖皮质激素分泌和时钟基因表达的影响。与正常饮食的小鼠相比，给予HFS饮食的小鼠在正常光/暗和恒定暗条件下显示出减少的运动活性。盲肠糖皮质激素分泌的昼夜节律和肾上腺中糖皮质激素相关基因和时钟基因的表达水平与HFS饮食中断。这些结果表明，HFS饮食改变运动活动，破坏昼夜节律的糖皮质激素分泌，并下调外周肾上腺生物钟基因。
Salt is an essential nutrient; however, excessive salt intake is a prominent public health concern worldwide. Various physiological functions are associated with circadian rhythms, and disruption of circadian rhythms is a prominent risk factor for cardiovascular diseases, cancer, and immune disease. Certain nutrients are vital regulators of peripheral circadian clocks. However, the role of a high-fat and high-salt (HFS) diet in the regulation of circadian gene expression is unclear. This study aimed to investigate the effect of an HFS diet on rhythms of locomotor activity, caecum glucocorticoid secretion, and clock gene expression in mice. Mice administered an HFS diet displayed reduced locomotor activity under normal light/dark and constant dark conditions in comparison with those administered a normal diet. The diurnal rhythm of caecum glucocorticoid secretion and the expression levels of glucocorticoid-related genes and clock genes in the adrenal gland were disrupted with an HFS diet. These results suggest that an HFS diet alters locomotor activity, disrupts circadian rhythms of glucocorticoid secretion, and downregulates peripheral adrenal gland circadian clock genes.