Three-dimensional in vitro reaggregates of embryonic cardiomyocytes:: A potential model system for monitoring effects of bioactive agents
Three-dimensional in vitro reaggregates of embryonic cardiomyocytes:: A potential model system for monitoring effects of bioactive agents
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DOI：
10.1177/1087057105280070
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发表时间：
2005-12-01
影响因子：
--
通讯作者：
Robitzki, A
中科院分区：
文献类型：
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作者：
Bartholomä, P;Gorjup, E;Robitzki, A
为了了解药物和治疗中使用的物质对心肌组织的生理作用，需要反映活体组织在体内情况的模型系统。因此，三维（3D）细胞聚集体的创建提供了一种改进和完善的体外模型，作为无细胞或单细胞与体内器官或整个生物体之间的联系。在这里，我们描述了一个稳定收缩的体外组织模型，它由胚胎鸡心肌细胞组成。为了建立基于细胞的检测系统，根据特殊心肌细胞类型的信使RNA表达和连接蛋白-43等功能标记物的蛋白表达模式，对体外三维心肌细胞球进行表征。最后，采用体外球体模型研究β -肾上腺素能受体激动剂异丙肾上腺素对配体受体相互作用介导的收缩性的影响。
To understand the physiological effects of substances used in drugs and therapies on heart muscle tissue, model systems that mirror the in vivo situation of living tissues are required. Therefore, the creation of 3-dimensional (3D) cell aggregates provides an improved and refined in vitro model as a link between cell-free or single cells and organs or whole organisms in vivo. Here we have characterized a stable contracting in vitro tissue model, which consists of embryonic chicken cardiomyocytes. For establishing a cell-based test system, the 3D in vitro cardiomyocyte spheres were characterized according to messenger RNA expression of special cardiac cell types and protein expression pattern of functional markers such as connexin-43. Finally, the in vitro spheroid model was used for investigating the effect of isoproterenol, a beta-adrenergic receptor agonist, on the contractibility mediated by the ligand receptor interaction.