Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro
Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro
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DOI：
10.1163/156856200743805
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发表时间：
2000-01-01
影响因子：
3.6
通讯作者：
Anseth, KS
中科院分区：
文献类型：
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作者：
Bryant, SJ;Nuttelman, CR;Anseth, KS
这项工作研究了几种潜在的细胞封装应用的光引发系统的细胞相容性。研究了紫外光和可见光引发方案。紫外光引发剂包括2,2-二甲氧基-2-苯基苯乙酮（Irgacure 651）、1-羟基环己基苯基酮（Irgacure 184）、2-甲基-1-[4-（甲基硫）苯基]-2-(4-morpholinyl)-1-propanone （Irgacure 907）和2-羟基-1-[4-（羟基乙氧基）苯基]-2-甲基-1-propanone （Darocur 2959）。以4-N - n -二甲氨基苯甲酸乙酯（4EDMAB）和三乙醇胺（TEA）组成的樟脑醌（CQ）和光敏剂异丙基硫氧蒽酮为可见光引发体系。培养成纤维细胞系NIH/3T3暴露于不同浓度的光引发剂中，浓度从0.01% （w/w）到0.1% (w/w)，有和没有引发光。结果表明，在低光引发剂浓度（小于或等于0.01% (w/w)）下，除CQ、Irgacure 651和4EDMAB的相对存活率比对照低50%外，所有引发剂分子都具有细胞相容性。在低强度引发光（类似于365 nm紫外光的6 mW cm（-2）和470-490 nm可见光的60 mW cm(-2)）和引发自由基的存在下，浓度小于或等于0.05% （w/w）的Darocur 2959和浓度小于或等于0.01% （w/w）的CQ分别是最有希望的细胞相容性紫外光和可见光引发系统。为了证明细胞相容性光启动系统在细胞包封应用中的潜在用途，将软骨细胞包封在使用0.05% (w/w) Darocur 2959（细胞相容性）和0.01% (w/w) Irgacure 651（细胞不相容）的光交联水凝胶中。在大约8mw cm（-2）的365 nm光下进行10分钟的光聚合后，几乎所有的软骨细胞都能在Darocur 2959的过程中存活，而Irgacure 651的过程中很少有软骨细胞存活。
This work investigates the cytocompatibility of several photoinitiating systems for potential cell encapsulation applications. Both UV and visible light initiating schemes were examined. The UV photoinitiators included 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651), 1-hydroxycyclohexyl phenyl ketone (Irgacure 184), 2-methyl-1-[4-(methylthio) phenyl]-2-(4-morpholinyl)-1-propanone (Irgacure 907), and 2-hydroxy-1-[4-(hydroxyethoxy)phenyl]-2-methyl-1-propanone (Darocur 2959). The visible light initiating systems included camphorquinone (CQ) with ethyl 4-N,N-dimethylaminobenzoate (4EDMAB) and triethanolamine (TEA) and the photosensitizer isopropyl thioxanthone. A cultured fibroblast cell line, NIH/3T3, was exposed to the photoinitiators at varying concentrations from 0.01% (w/w) to 0.1% (w/w) with and without the presence of initiating light. The results demonstrated that at low photoinitiator concentrations (less than or equal to 0.01% (w/w)), all of the initiator molecules were cytocompatible with the exception of CQ, Irgacure 651, and 4EDMAB which had a relative survival similar to 50% lower than a control. In the presence of low intensity initiating light (similar to 6 mW cm(-2) of 365 nm UV light and similar to 60 mW cm(-2) of 470-490 nm visible light) and initiating radicals, Darocur 2959 at concentrations less than or equal to 0.05% (w/w) and CQ at concentrations less than or equal to 0.01% (w/w) were the most promising cytocompatible UV and visible light initiating systems, respectively. To demonstrate the potential use of cytocompatible photoinitiating systems in cell encapsulation applications, chondrocytes were encapsulated in a photocrosslinked hydrogel using 0.05% (w/w) Darocur 2959 (cytocompatible) and 0.01% (w/w) Irgacure 651 (cyto-incompatible). After photopolymerizing for 10 minutes with similar to 8 mW cm(-2) of 365 nm light, nearly all the chondrocytes survived the process with Darocur 2959 while very few of the chondrocytes survived the process with Irgacure 651.