Use of Lignin Extracted from Different Plant Sources as Standards in the Spectrophotometric Acetyl Bromide Lignin Method
Use of Lignin Extracted from Different Plant Sources as Standards in the Spectrophotometric Acetyl Bromide Lignin Method
复制标题
DOI：
10.1021/jf104826n
复制
发表时间：
2011-04-27
影响因子：
6.1
通讯作者：
Kerley, Monty S.
中科院分区：
文献类型：
--
作者：
Fukushima, Romualdo S.;Kerley, Monty S.
非重量法乙酰溴木质素（ABL）的方法进行了评估，以量化木质素的浓度在各种植物材料。传统的木质素定量方法需要用酸性二氧六环提取木质素，并从每个植物样品中分离木质素，通过分光光度分析构建标准曲线。然后测量预提取的植物细胞壁中的木质素浓度。然而，这提出了一个方法的复杂性，因为提取和分离程序是漫长和繁琐的，特别是如果有许多样本涉及。这项工作旨在简化木质素定量。我们的假设是，任何木质素，无论其植物来源，可以用来构建一个标准曲线的目的，确定木质素浓度在各种植物。为了验证我们的假设，从一系列不同的植物中分离木质素，并与三种商业木质素一起沿着，建立标准曲线并在它们之间进行比较。从这些标准曲线得到的斜率和截距足够接近，可以利用回归方程中的平均消光系数来估计：任何植物中的木质素浓度，与其植物来源无关。通过使用共同的回归方程来定量木质素，避免了木质素提取、分离和标准曲线构建的步骤，这大大加快了ABL方法。乙酰溴木质素法是一种快速、方便的木质素定量分析方法。
A nongravimetric acetyl bromide lignin (ABL) method was evaluated to quantify lignin concentration in a variety of plant materials. The traditional approach to lignin quantification required extraction of lignin with acidic dioxane and its isolation from each plant sample to construct a standard curve via spectrophotometric analysis. Lignin concentration was then measured in pre-extracted plant cell walls. However, this presented a methodological complexity because extraction and isolation procedures are lengthy and tedious, particularly if there are many samples involved. This work was targeted to simplify lignin quantification. Our hypothesis was that any lignin, regardless of its botanical origin, could be used to construct a standard curve for the purpose of determining lignin concentration in a variety of plants. To test our hypothesis, lignins were isolated from a range of diverse plants and, along with three commercial lignins, standard curves were built and compared among them. Slopes and intercepts derived from these standard curves were close enough to allow utilization of a mean extinction coefficient in the regression equation to estimate : lignin concentration in any plant, independent of its botanical origin. Lignin quantification by use of a common regression equation obviates the steps of lignin extraction, isolation, and standard curve construction, which substantially expedites the ABL method. Acetyl bromide lignin method is a fast, convenient analytical procedure that may routinely be used to quantify lignin.