Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex
Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex
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DOI：
10.1016/j.devcel.2016.09.028
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发表时间：
2016-10
影响因子：
11.8
通讯作者：
C. Oliva;A. Soldano;Natalia Mora;N. de Geest;Annelies Claeys;Maria-Luise Erfurth;J. Sierralta;A. Ramaekers;Dan Dascenco;R. Ejsmont;D. Schmucker;N. Sánchez-Soriano;Bassem A. Hassan
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文献类型：
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作者：
C. Oliva;A. Soldano;Natalia Mora;N. de Geest;Annelies Claeys;Maria-Luise Erfurth;J. Sierralta;A. Ramaekers;Dan Dascenco;R. Ejsmont;D. Schmucker;N. Sánchez-Soriano;Bassem A. Hassan
轴突布线分子Slit及其周围（Robo）受体是神经索模式的保守调节因子。机器人感受器也有助于连接大脑回路。调节这些信号的分子机制是否被修改以适应更复杂的大脑连接过程尚不清楚。我们研究了Slit和Robo受体在果蝇高阶脑回路中的作用，并确定了Robo/Slit功能在细胞和分子机制上的差异。首先，我们发现大脑中Robo受体的信号是由受体蛋白酪氨酸磷酸酶RPTP69d调节的。RPTP69d在不影响其磷酸化状态的情况下增加了Robo3的膜可用性。其次，我们在大脑发育过程中没有检测到Slit的中线定位。相反，Slit富含蘑菇体，这是一种覆盖大脑大片区域的神经元结构。因此，一种不同的分子机制调节果蝇大脑中的神经元回路，部分是对来自蘑菇体的信号作出反应。
The axonal wiring molecule Slit and its Round-About (Robo) receptors are conserved regulators of nerve cord patterning. Robo receptors also contribute to wiring brain circuits. Whether molecular mechanisms regulating these signals are modified to fit more complex brain wiring processes is unclear. We investigated the role of Slit and Robo receptors in wiringDrosophilahigher-order brain circuits and identified differences in the cellular and molecular mechanisms of Robo/Slit function. First, we find that signaling by Robo receptors in the brain is regulated by the Receptor Protein Tyrosine Phosphatase RPTP69d. RPTP69d increases membrane availability of Robo3 without affecting its phosphorylation state. Second, we detect no midline localization of Slit during brain development. Instead, Slit is enriched in the mushroom body, a neuronal structure covering large areas of the brain. Thus, a divergent molecular mechanism regulates neuronal circuit wiring in theDrosophilabrain, partly in response to signals from the mushroom body.