NESH (Abi-3) is pre-sent in the Abi/WAVE complex but does not promote c-Abl-mediated phosphorylation
NESH (Abi-3) is pre-sent in the Abi/WAVE complex but does not promote c-Abl-mediated phosphorylation
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DOI：
10.1016/j.febslet.2006.10.065
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发表时间：
2006-11-27
期刊：
影响因子：
3.5
通讯作者：
Tani, Katsuko
中科院分区：
文献类型：
--
作者：
Hirao, Noriko;Sato, Seiichi;Tani, Katsuko
Abl相互作用因子（Abi）被鉴定为Abl酪氨酸激酶结合蛋白，随后被证明是大分子Abi/WAVE复合物的一个组成部分，该复合物是rae依赖性肌动蛋白聚合的关键调节因子。先前的研究表明，Abi-1促进c- b1介导的哺乳动物激活（Mena）和WAVE2的磷酸化。除了Abi-1，哺乳动物还具有Abi-2和NESH （Abi-3）。在这项研究中，我们比较了三种Abi蛋白在促进c- abl介导的磷酸化和Abi/WAVE复合物形成方面的作用。虽然Abi-2和Abi-1一样，促进了c- abl介导的Mena和WAVE2的磷酸化，但NESH （Abi-3）没有这种作用。这种差异可能是由于它们对c-Abl的结合能力。免疫沉淀显示Abi/WAVE复合体中存在NESH （Abi-3）。我们的研究结果表明，与Abi-1和Abi-2一样，NESH （Abi-3）是Abi/WAVE复合体的一个组成部分，但可能在c-Abl的调节中起不同的作用。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
Abl interactor (Abi) was identified as an Abl tyrosine kinase-binding protein and subsequently shown to be a component of the macromolecular Abi/WAVE complex, which is a key regulator of Rae-dependent actin polymerization. Previous studies showed that Abi-1 promotes c-Ab1-mediated phosphorylation of Mammalian Enabled (Mena) and WAVE2. In addition to Abi-1, mammals possess Abi-2 and NESH (Abi-3). In this study, we compared the three Abi proteins in terms of the promotion of c-Abl-mediated phosphorylation and the formation of Abi/WAVE complex. Although Abi-2, like Abi-1, promoted the c-Abl-mediated phosphorylation of Mena and WAVE2, NESH (Abi-3) had no such effect. This difference was likely due to their binding abilities as to c-Abl. Immunoprecipitation revealed that NESH (Abi-3) is present in the Abi/WAVE complex. Our results suggest that NESH (Abi-3), like Abi-1 and Abi-2, is a component of the Abi/WAVE complex, but likely plays a different role in the regulation of c-Abl. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.