Organization and cellular arrangement of two neurogenic regions in the adult ferret (Mustela putorius furo) brain.
Organization and cellular arrangement of two neurogenic regions in the adult ferret (Mustela putorius furo) brain.
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成年雪貂 (Mustela putorius furo) 大脑中两个神经源区域的组织和细胞排列。
DOI：
10.1002/cne.23503
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yamada H
中科院分区：
文献类型：
--
作者：
Takamori Y;Wakabayashi T;Mori T;Kosaka J;Yamada H
在成年哺乳动物的大脑中，已经确定了两个神经发生区，即侧脑室（LV）的室下区（SVZ）和齿状回（DG）的亚颗粒区（SGZ）。尽管我们对啮齿类动物了解甚多，但对大多数哺乳动物神经源性区域的详细排列却知之甚少。在这项研究中，我们使用免疫组织化学和细胞类型特异性抗体来研究成年雪貂的两个生发区组织，雪貂属于食肉目，被广泛用作具有回头脑的模型动物。从SVZ到嗅球，双皮质素阳性细胞倾向于以链状簇状组织，这些簇状簇被星形胶质细胞网络包围。这种结构与其他物种中描述的吻侧迁移流（RMS）相似。与啮齿类动物不同的是，RMS的水平肢直接从左室伸出，左室前区向喙侧延伸并到达嗅球。在DG中，具有长放射状突起的胶质纤维酸性蛋白阳性细胞以及双皮质素阳性细胞在SGZ中定向。在这两个区域，双皮质素阳性细胞表现出特有的形态，并且多唾液化神经细胞粘附分子、β - III微管蛋白和层粘连蛋白B1（强染色）呈阳性。使用针对增殖细胞核抗原和磷酸组蛋白H3的抗体在两个区域检测到增殖细胞。这些观察结果表明，尽管雪貂的大脑在解剖学上存在差异，但雪貂的两个神经发生区域具有与其他哺乳动物相似的细胞组成。中国生物医学工程学报，2014,22(2):518 - 538。©2013 Wiley期刊公司
In the adult mammalian brain, two neurogenic regions have been characterized, the subventricular zone (SVZ) of the lateral ventricle (LV) and the subgranular zone (SGZ) of the dentate gyrus (DG). Despite remarkable knowledge of rodents, the detailed arrangement of neurogenic regions in most mammals is poorly understood. In this study, we used immunohistochemistry and cell type‐specific antibodies to investigate the organization of two germinal regions in the adult ferret, which belongs to the order Carnivora and is widely used as a model animal with a gyrencephalic brain. From the SVZ to the olfactory bulb, doublecortin‐positive cells tended to organize in chain‐like clusters, which are surrounded by a meshwork of astrocytes. This structure is homologous to the rostral migratory stream (RMS) described in other species. Different from rodents, the horizontal limb of the RMS emerges directly from the LV, and the anterior region of the LV extends rostrally and reached the olfactory bulb. In the DG, glial fibrillary acidic protein‐positive cells with long radial processes as well as doublecortin‐positive cells are oriented in the SGZ. In both regions, doublecortin‐positive cells showed characteristic morphology and were positive for polysialylated‐neural cell adhesion molecule, beta‐III tubulin, and lamin B1 (intense staining). Proliferating cells were detected in both regions using antibodies against proliferating cell nuclear antigen and phospho‐histone H3. These observations demonstrate that the two neurogenic regions in ferrets have a similar cellular composition as those of other mammalian species despite anatomical differences in the brain. J. Comp. Neurol. 522:1818–1838, 2014. © 2013 Wiley Periodicals, Inc.