1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen
1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen
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DOI：
10.1021/es501601w
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发表时间：
2014-06-17
影响因子：
11.4
通讯作者：
Widdicombe, Steve
中科院分区：
文献类型：
--
作者：
Ellis, Robert P.;Spicer, John I.;Widdicombe, Steve
人类活动正在从根本上改变世界海洋的化学成分。海洋酸化是在气候变暖和疾病暴发增多的背景下发生的，对海洋生物、群落和生态系统构成重大威胁。在目前的研究中，利用H-1核磁共振波谱研究了紫贻贝对海水pH降低和温度升高90天以及随后的致病攻击的反应。对代谢物的分析表明，雄性和雌性生物体之间存在显著差异。此外，男性和女性对环境压力的反应也不同。虽然雄性受到海水pH值降低、温度升高和细菌挑战的显著影响，但只有海水pH值的下降才会影响雌性。尽管对男性和女性的影响不同，但应激源似乎是通过一种影响两性能量代谢和渗透平衡的全面性应激反应来发挥作用的。因此，这项研究对于解释贻贝的代谢数据以及环境胁迫对海洋无脊椎动物的影响具有重要的意义。
Human activities are fundamentally altering the chemistry of the world's oceans. Ocean acidification (OA) is occurring against a background of warming and an increasing occurrence of disease outbreaks, posing a significant threat to marine organisms, communities, and ecosystems. In the current study, H-1 NMR spectroscopy was used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater pH and increased temperature, followed by a subsequent pathogenic challenge. Analysis of the metabolome revealed significant differences between male and female organisms. Furthermore, males and females are shown to respond differently to environmental stress. While males were significantly affected by reduced seawater pH, increased temperature, and a bacterial challenge, it was only a reduction in seawater pH that impacted females. Despite impacting males and females differently, stressors seem to act via a generalized stress response impacting both energy metabolism and osmotic balance in both sexes. This study therefore has important implications for the interpretation of metabolomic data in mussels, as well as the impact of environmental stress in marine invertebrates in general.