A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems
A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems
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DOI：
10.1002/ppap.201600199
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发表时间：
2017-08
影响因子：
3.5
通讯作者：
J. Lackmann;M. Fiebrandt;M. Raguse;K. Kartaschew;M. Havenith;J. Bandow;R. Moeller;P. Awakowicz;K. Stapelmann
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文献类型：
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作者：
J. Lackmann;M. Fiebrandt;M. Raguse;K. Kartaschew;M. Havenith;J. Bandow;R. Moeller;P. Awakowicz;K. Stapelmann
低压等离子体是现代灭菌工艺的一种有前途的替代方法。由于等离子体是一个表面过程，孢子的多层堆叠是一个需要克服的关键挑战。在这里，分析了冷凝过氧化氢和氢等离子体的组合工艺对各种孢子浓度的功效，与单独使用的两个步骤相比，使用组合工艺的功效明显增加。除孢子外，蛋白质污染是临床上的主要问题，并且使用良好建立的BSA模型研究组合工艺的蛋白质去除效率。此外，RNase A作为一种难以纯化的蛋白质模型来研究蛋白质失活效率。最后，RNase A的失活机制，特别侧重于硫基修饰的研究，使用拉曼光谱。
Low-pressure plasmas are a promising alternative to modern sterilization processes. As plasma is a surface process, multilayered stacks of spores are a crucial challenge to overcome. Here, a combined process of condensed hydrogen peroxide and hydrogen plasma is analyzed for its efficacy against various spore concentrations showing a clear increase in efficacy using a combined process compared to the two steps used separately. Besides spores, protein contaminations are a major issue in clinics and the combined process is investigated for protein removal efficiency using the well-established BSA model. Furthermore, RNase A serves as a difficult-to-inactivate protein model to investigate protein inactivation efficiency. Finally, inactivation mechanisms of RNase A with a special focus on sulfur-based modifications are investigated using Raman spectroscopy.