Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8
Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8
复制标题
使用针对转录因子 L3/Lhx8 的 RNAi 调节和/或抑制小鼠胚胎干细胞的胆碱能分化
DOI：
10.1007/978-1-60761-769-3_8
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发表时间：
2010
影响因子：
--
通讯作者：
Wanaka A
中科院分区：
文献类型：
--
作者：
Manabe T;Wanaka A
控制胚胎干细胞中特定基因表达的技术对于阐明该基因的功能是有效和重要的。关于细胞分化成神经系统成分，这些技术将发挥关键作用，不仅阐明神经元和神经胶质细胞的分化机制，而且还如何确定神经元表型。在这一章中，我们描述了一种RNA干扰方法，通过敲低转录因子L3/Lhx 8（一种Lim同源异型盒基因家族蛋白）的表达来抑制小鼠胚胎干细胞的胆碱能分化。这种方法将极大地促进参与神经元分化和再生的因素的功能分析，并将有助于细胞移植研究。
Techniques for controlling the expression of a specific gene in embryonic stem cells are effective and important for clarifying the functions of the gene. Regarding differentiation of cells into nervous system components, these techniques would play key roles in elucidating, not only the differentiation mechanisms of neuronal and glial cells but also how neuronal phenotypes are determined. In this chapter, we describe a RNA interference method for suppressing cholinergic differentiation in murine embryonic stem cells by knockdown of expression of the transcription factor L3/Lhx8, a Lim homeobox gene family protein. This method will greatly facilitate functional analyses of the factors involved in neuronal differentiation and regeneration and will contribute to cell transplantation studies.