Pnolezoelectric ink-jet printing of horseradish peroxidase: Effect of ink viscosity modifiers on activity
Pnolezoelectric ink-jet printing of horseradish peroxidase: Effect of ink viscosity modifiers on activity
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DOI：
10.1002/marc.200700226
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发表时间：
2007-09-18
影响因子：
4.6
通讯作者：
Yan, Ning
中科院分区：
文献类型：
--
作者：
Di Risio, Sabina;Yan, Ning
在这项研究中，含有辣根过氧化物酶（HRP）酶的生物墨水配制和喷射与压电基喷墨沉积系统。测试了具有不同分子量、官能团和电荷的许多聚合物作为粘度改性剂。系统地研究了粘度调节剂对HRP活性的影响。发现通常用于商业油墨制剂中的粘度调节剂显著损害HRP活性，这可能是由于扩散限制。相比之下，羧甲基纤维素将粘度增加到压电喷射所需的范围，而不会对HRP活性产生负面影响。通过添加合适的表面活性剂、粘度调节剂和保湿剂，使HRP生物墨水获得良好的润湿性。
In this study a bioink containing the horseradish peroxidase (HRP) enzyme was formulated and jetted with a piezoelectric-based ink-jet deposition system. A number of polymers with different molecular weights, functional groups and electric charge were tested as viscosity modifiers. The impact of viscosity modifiers on HRP activity was systematically studied. It was found that viscosity modifiers typically used in commercial ink formulations significantly impaired HRP activity possibly due limitations diffusion. contrast, carboxymethyl cellulose increased viscosity to the desired range for piezoelectric jetting without negatively affecting the HRP activity. Good jettability was obtained for the HRP bioink by adding a suitable surfactant, a viscosity modifier and a humectant.