SYNCRIP (synaptotagmin-binding, cytoplasmic RNA-interacting protein) is a host factor involved in hepatitis C virus RNA replication.
SYNCRIP (synaptotagmin-binding, cytoplasmic RNA-interacting protein) is a host factor involved in hepatitis C virus RNA replication.
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DOI：
10.1016/j.virol.2009.01.018
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发表时间：
2009-04-10
期刊：
影响因子：
3.7
通讯作者：
Lai, Michael M. C.
中科院分区：
文献类型：
--
作者：
Liu, Helene Minyi;Aizaki, Hideki;Choi, Keum S.;Machida, Keigo;Ou, James J. -H.;Lai, Michael M. C.
丙型肝炎病毒 (HCV) RNA 复制需要病毒非结构蛋白和细胞因子。最近，一种细胞蛋白突触结合蛋白结合细胞质 RNA 相互作用蛋白 (SYNCRIP)，也称为 NSAP1，被发现可以结合 HCV RNA 并增强 HCV IRES 依赖性翻译。我们研究该蛋白是否也参与 HCV RNA 复制。我们发现 SYNCRIP 与耐去垢剂膜组分相关，并与新合成的 HCV RNA 共定位。 siRNA 敲低 SYNCRIP 显着降低了含有亚基因组复制子或全长病毒 RNA 的细胞中 HCV RNA 的量。最后，SYNCRIP 免疫耗竭后的体外复制测定表明，SYNCRIP 直接参与 HCV RNA 复制。这些发现表明SYNCRIP具有双重功能，参与HCV生命周期中的RNA复制和翻译。
Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whether this protein is also involved in the HCV RNA replication. We found that SYNCRIP was associated with detergent-resistant membrane fractions and colocalized with newly-synthesized HCV RNA. Knock-down of SYNCRIP by siRNA significantly decreased the amount of HCV RNA in the cells containing a subgenomic replicon or a full-length viral RNA. Lastly, an in vitro replication assay after immunodepletion of SYNCRIP showed that SYNCRIP was directly involved in HCV RNA replication. These findings indicate that SYNCRIP has dual functions, participating in both RNA replication and translation in HCV life cycle.
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