Expression of idiotype on the surface of human B cells producing anti-DNA antibody.
Expression of idiotype on the surface of human B cells producing anti-DNA antibody.
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产生抗DNA抗体的人B细胞表面独特型的表达。
DOI：
10.4049/jimmunol.136.4.1241
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发表时间：
1986
影响因子：
4.4
通讯作者：
K. Yoshinaga
中科院分区：
文献类型：
--
作者：
E. Tamate;T. Sasaki;T. Muryoi;O. Takai;K. Otani;K. Tada;K. Yoshinaga
我们分析了人抗DNA抗体产生细胞表面的独特型（Id）表达。制备了鼠单克隆抗Id抗体，其对与人单克隆抗DNA自身抗体的抗原结合位点相关的决定簇具有特异性。一种抗Id抗体仅与抗ssDNA产生细胞上的表面Id反应，但不与抗dsDNA产生B细胞克隆上的表面Id反应。另一种抗Id抗体确实结合抗dsDNA克隆上的表面Id，但不结合抗ssDNA克隆上的表面Id。通过用DNA或适当的多核苷酸抗原预处理克隆，或通过用游离抗DNA抗体预吸收抗Id抗体，抑制抗Id和表面Id之间的相互作用。表面IgM和IgD表达与从克隆分泌的抗体相同的Id。用抗Id抗体处理Id阳性克隆可诱导细胞表面Id的重新分布，表明这些细胞是抗Id抗体调节作用的靶细胞。
We analyzed the idiotype (Id) expression on the surface of human anti-DNA antibody-producing cells. Murine monoclonal anti-Id antibodies with a specificity for determinants associated with the antigen-binding sites of human monoclonal anti-DNA autoantibodies were prepared. One anti-Id antibody reacted only with surface Id on anti-ssDNA-producing cells, but not with those on anti-dsDNA-producing B cell clones. Another anti-Id antibody did bind the surface Id on anti-dsDNA clones, but not those on anti-ssDNA clones. The interaction between anti-Id and surface Id was inhibited by pretreatment of the clones with DNA or appropriate polynucleotide antigens, or by preabsorption of anti-Id antibodies with free anti-DNA antibodies. Surface IgM and IgD expressed the same Id as the antibody secreted from the clones. The treatment of Id-positive clones by anti-Id antibody induced the redistribution of surface Id on the cells, indicating that these cells serve as targets for the regulatory action of anti-Id antibody.