The functional impact of Pgm amino acid polymorphism on glycogen content in Drosophila melanogaster.
The functional impact of Pgm amino acid polymorphism on glycogen content in Drosophila melanogaster.
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Pgm 氨基酸多态性对果蝇糖原含量的功能影响。
DOI：
10.1093/genetics/159.1.201
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发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Eanes,WF
中科院分区：
文献类型：
--
作者：
Verrelli,BC;Eanes,WF
早期对果蝇中常见的PGM等位酶的研究没有报道体外活性差异。然而，我们对核苷酸变异的研究发现，PGM等位酶是氨基酸多态性的异质混合物。在这项研究中，我们分析了10个PGM蛋白单倍型方面的PGM活性，热稳定性，和成人糖原含量。我们发现PGM蛋白单倍型之间的活性存在两倍差异，这与糖原含量的三倍差异有关。几个PGM氨基酸多态性的纬度梯度表明，高PGM活性和明显较高的糖原合成通量，与北方纬度戊糖分流通量减少的G6 PD低活性梯度平行。这表明，氨基酸多态性是在选择在这个分支点，并可能有利于增加代谢储存与胁迫抗性和适应温带地区。
Earlier studies of the common PGM allozymes inDrosophila melanogasterreported noin vitroactivity differences. However, our study of nucleotide variation observed that PGM allozymes are a heterogeneous mixture of amino acid polymorphisms. In this study, we analyze 10 PGM protein haplotypes with respect to PGM activity, thermostability, and adult glycogen content. We find a twofold difference in activity among PGM protein haplotypes that is associated with a threefold difference in glycogen content. The latitudinal clines for severalPgmamino acid polymorphisms show that high PGM activity, and apparently higher flux to glycogen synthesis, parallel the low activity clines at G6PD for reduced pentose shunt flux in northern latitudes. This suggests that amino acid polymorphism is under selection at this branch point and may be favored for increased metabolic storage associated with stress resistance and adaptation to temperate regions.