Automated recording of vertical negative phototactic behaviour in Daphnia magna straus (Crustacea)
Automated recording of vertical negative phototactic behaviour in Daphnia magna straus (Crustacea)
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DOI：
10.1007/s10750-005-1259-1
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发表时间：
2006-04-01
期刊：
影响因子：
2.6
通讯作者：
Schmidt, S
中科院分区：
文献类型：
--
作者：
Gerhardt, A;de Bisthoven, LJ;Schmidt, S
在浮游动物的昼夜活动节律中，昼夜垂直迁移是一个众所周知的现象。我们的目的是测试大水蚤（Daphnia magna）克隆喙的负趋光性（由人工诱导的光胁迫、每隔2 h的明暗交替引起）及其对内源性垂直迁移节律的干扰是否可以通过生物监测仪自动记录。在全自动生物测试系统（multi - species Freshwater Biomonitor）中，首次定量记录了D. magna的垂直游动行为，该系统采用非光学数据记录。该实验装置由三个记录单元组成（3层室）。在3层腔室中可以清楚地记录昼夜垂直迁移，5层腔室比1层腔室的节奏更清晰。生物明显响应诱导光胁迫负趋光性，但最好在较大的室。人工诱导的节律受内源节律的影响。该方法可为今后浮游动物垂直游动活动的长期观测提供便利。
Diurnal vertical migration is a well-known phenomenon in the circadian activity rhythms of zooplankton. Our goal was to test whether negative phototaxis in Daphnia magna clone BEAK (provoked by artificially induced light stress, alternating light and dark phases in 2 h intervals), and its interference with the endogenous rhythm of diurnal vertical migration, can be automatically registered with a biomonitor. For the first time the vertical swimming behaviour of D. magna was recorded quantitatively based on non-optical data recording in a fully automated biotest system, the Multispecies Freshwater Biomonitor in a new experimental setup consisting of a column of three recording units (3-level chambers). Circadian vertical migration was clearly recorded in the 3-level chambers and the rhythm was more clear with 5 than with 1 organism per chamber. The organisms clearly responded to induced light stress with negative phototaxis, however best in larger chambers. The artificially induced rhythm was influenced by the endogenous rhythm. This approach may facilitate long-term observations of vertical swimming activity of zooplankton in the future.