Identification of the M-CSF receptor in endometriosis by immunohistochemistry and RT-PCR
Identification of the M-CSF receptor in endometriosis by immunohistochemistry and RT-PCR
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DOI：
10.1111/j.1600-0897.2004.00228.x
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发表时间：
2004-11-01
影响因子：
3.6
通讯作者：
Salmassi, A
中科院分区：
文献类型：
--
作者：
Mettler, L;Schmutzler, AG;Salmassi, A
问题：本文的目的是提供进一步的证据表明，异位增生上皮细胞的刺激引起的腹腔巨噬细胞。这是必要的，以显示增生性上皮细胞表达的巨噬细胞集落刺激因子受体（M-CSFR）结合的M-CSF产生的腹腔macrophage. Method的研究：为检测M-CSFR，异位子宫内膜（n = 79）和在位子宫内膜（n = 18）的样品进行了比较。在腹腔镜手术中获取周期增殖期的标本。冰冻切片用于免疫组织化学检测。对于体外逆转录酶聚合酶链反应（RT-PCR）试验，将组织立即在石蜡切片上休克冷冻。对于原位RT-PCR技术，将标本置于多聚甲醛溶液中，包埋在石蜡中，随后进行处理。结果：M-CSF及其受体在在位和异位子宫内膜中均有表达。定性地，用两种PCR技术，我们发现M-CSF受体存在于所有检查的样品中。使用组织化学检测技术，M-CSF受体被发现在近70%的子宫内膜异位症患者相比，一个统计学上显着较低的百分比在正常endometrium.CONCLUSIONS：原位RT-PCR技术和免疫组化阐述了需要跟踪的细胞来源的M-CSF受体。M-CSF受体在子宫内膜异位症组织和子宫内膜中的鉴定，为子宫内膜异位症的研究开辟了新的实验领域。
PROBLEM: The aim of this paper is to provide further evidence that the dystopic proliferation of endometriotic epithelia is caused by the stimulation of peritoneal macrophages. It is essential to show that endometriotic epithelial cells express the macrophage colony-stimulating factor receptor (M-CSFR) which binds the M-CSF produced by the peritoneal macrophages.METHOD OF STUDY: For the detection of M-CSFR, samples of ectopic endometrium (n = 79) and eutopic endometrium (n = 18) were compared. The specimens were gained at operative laparoscopy in the proliferative phase of the cycle. Cryostat sections were used for immunohistochemical detection. For in vitro reverse transcriptase polymerase chain reaction (RT-PCR) tests, the tissue was immediately shock frozen on paraffin sections. For the in situ RT-PCR technique the specimens were placed in a para-formaldehyde solution, embedded in paraffin and later processed. The Gene Amp 1000 in situ PCR system (Perkin Elmer) was used as the thermal cycler.RESULTS: M-CSF and the M-CSF receptor are present in eutopic and ectopic endometrium. Qualitatively, with both PCR techniques we found the M-CSF receptor to be present in all samples examined. Using the histochemical detection technique, the M-CSF receptor was found in nearly 70% of endometriosis patients compared with a statistically significant lower percentage in normal endometrium.CONCLUSIONS: The in situ RT-PCR technique and immunohistochemistry elaborated the need to trace the cellular sources of the M-CSF receptor. The identification of the M-CSF receptor in endometriotic tissue and in endometrium is apt to open a new experimental field in endometriosis research.