No significant difference in antigenicity or tissue transglutaminase substrate specificity of Irish and US wheat gliadins.
No significant difference in antigenicity or tissue transglutaminase substrate specificity of Irish and US wheat gliadins.
复制标题
爱尔兰和美国小麦麦醇溶蛋白的抗原性或组织转谷氨酰胺酶底物特异性没有显着差异。
DOI：
10.1023/a:1005404012558
复制
发表时间：
2000
影响因子：
3.1
通讯作者：
Nunes,DP
中科院分区：
文献类型：
--
作者：
Keaveny,AP;Offner,GD;Bootle,E;Nunes,DP
临床乳糜泻的患病率已被证明随时间和遗传相似的人群而变化。小麦抗原性和转氨酶底物性质的差异是这些差异的可能解释。本研究评估了来自乳糜泻高发和低发地区的麦醇溶蛋白的抗原性和转氨酶底物特异性。麦醇溶蛋白是从三个商业美国小麦来源和两个爱尔兰来源中提取的。SDS-PAGE和蛋白质印迹法显示麦醇溶蛋白提取物中的微小但显著的变化。然而，ELISA显示这些醇溶蛋白的抗原性没有差异。谷氨酰胺转移酶预处理对醇溶蛋白的抗原性无明显影响，动力学研究表明，各种醇溶蛋白的Km值非常相似。纯化的伊加和IgG对转氨酶活性无影响。总之，小麦醇溶蛋白的微小变化不太可能解释在遗传相似的群体中观察到的疾病表达差异。
The prevalence of clinical celiac disease has been shown to vary both across time and between genetically similar populations. Differences in wheat antigenicity and transglutaminase substrate properties are a possible explanation for these differences. This study assessed the antigenicity and transglutaminase substrate specificities of gliadins from regions of high and low celiac disease prevalence. Gliadin was extracted from three commercial US wheat sources and two Irish sources. SDS-PAGE and western blotting revealed minor, but significant variations in the gliadin extracts. However, ELISA showed no difference in the antigenicity of these gliadins. Transglutaminase pretreatment of gliadin resulted in no significant change in gliadin antigenicity and kinetic studies showed that theKms of the various gliadins were very similar. Purified IgA and IgG had no effect on transglutaminase activity. In summary, minor variations in wheat gliadins are unlikely to explain the observed differences in disease expression across genetically similar populations.