MEDIATION OF EPSTEIN-BARR-VIRUS EBNA2 TRANSACTIVATION BY RECOMBINATION SIGNAL-BINDING PROTEIN J(K)
MEDIATION OF EPSTEIN-BARR-VIRUS EBNA2 TRANSACTIVATION BY RECOMBINATION SIGNAL-BINDING PROTEIN J(K)
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DOI：
10.1126/science.8016657
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发表时间：
1994-07-01
期刊：
影响因子：
56.9
通讯作者：
PETERSON, MG
中科院分区：
文献类型：
--
作者：
HENKEL, T;LING, PD;PETERSON, MG
Epstein-Barr 病毒 (EBV) 反式激活蛋白，称为 Epstein-Barr 病毒核抗原 2 (EBNA2)，在调节潜伏病毒转录和 EBV 感染的 B 细胞的永生化中发挥着关键作用。与大多数转录因子不同，EBNA2 不直接与其顺式反应 DNA 元件结合，而是需要一个细胞因子，称为 C 启动子结合因子 1 (CBF1)。在这里，CBF1 被纯化并被发现与 EBNA2 直接相互作用。 CBF1 与被认为参与免疫球蛋白基因重排的蛋白质 RBPJ(k)appa 相同。与之前的报道相反，CBF1-RBPJ(k)appa 不与重组信号序列结合，而是与 EBV C 启动子和 CD23 启动子中的位点结合。
The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJ(k)appa. Contrary to previous reports, CBF1-RBPJ(k)appa did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter.